Carpenè E
Boll Soc Ital Biol Sper. 1982 Aug 30;58(16):1005-11.
Ovis aries masseter is a typical muscle consisting of slow-twitch fibres. The muscle homogeneity is well evidenced histochemically by the myosin ATPase reaction. The extraction and purification of myosin from sheep masseter is reported. After its centrifugation and dialysis followed by ion-exchange chromatography, the protein analysed on SDS polyacrylamide gels showed the usual pattern of slow myosin. The light-chain LC2 was present as a double band, a fact which has already been reported and which could be ascribed to SH group oxidation. From the present data sheep masseter can be considered a good source of slow myosin, and we have used it satisfactorily in the preparation of specific antibodies.
绵羊咬肌是由慢肌纤维组成的典型肌肉。通过肌球蛋白ATP酶反应，在组织化学上充分证明了该肌肉的同质性。本文报道了从绵羊咬肌中提取和纯化肌球蛋白的方法。经过离心、透析，然后进行离子交换色谱法，在SDS聚丙烯酰胺凝胶上分析的蛋白质显示出慢肌球蛋白的常见模式。轻链LC2以双链形式存在，这一事实已经有报道，可能归因于SH基团氧化。根据目前的数据，绵羊咬肌可被认为是慢肌球蛋白的良好来源，并且我们已成功地将其用于制备特异性抗体。
