T 细胞前淋巴细胞白血病的免疫表型特征。
Immunophenotypic characterization of T-cell prolymphocytic leukemia.
机构信息
Dept of Laboratory Medicine, University of Washington, 1959 NE Pacific St, Seattle, WA 98195;
出版信息
Am J Clin Pathol. 2013 Nov;140(5):727-35. doi: 10.1309/AJCPG71KYOXTKLQW.
OBJECTIVES
To review clinical data, cytogenetic findings, and flow cytometric analysis in 20 patients with T-cell prolymphocytic leukemia (T-PLL), a rare, aggressive, mature T-cell leukemia with poor prognosis and short survival.
METHODS
Using multiparameter flow cytometry with a large combination of antibodies, we summarize the immunophenotypic features of T-PLL, including unusual immunophenotypic variants, and illustrate immunophenotypic clues that may help distinguish this entity from other T-cell malignancies.
RESULTS
By flow cytometry, T-PLL is characterized by a postthymic mature T-cell immunophenotype with a variety of abnormalities that usually allow distinction from other mature T-cell leukemias.
CONCLUSIONS
Although definitive diagnosis of T-PLL requires a systemic approach with integration of clinical data, morphology, immunophenotype, cytogenetics/fluorescence in situ hybridization, and molecular features, our results indicate immunophenotyping by multiparameter flow cytometry greatly facilitates diagnosis and assists with subclassification of this mature T-cell leukemia.
目的
回顾 20 例 T 细胞前淋巴细胞白血病（T-PLL）患者的临床资料、细胞遗传学发现和流式细胞分析结果。T-PLL 是一种罕见的侵袭性成熟 T 细胞白血病，预后差，生存期短。
方法
采用多参数流式细胞术和大量抗体组合，总结 T-PLL 的免疫表型特征，包括不常见的免疫表型变异，并阐明可能有助于将该疾病与其他 T 细胞恶性肿瘤相鉴别的免疫表型线索。
结果
通过流式细胞术，T-PLL 的特征是具有多种异常的胸腺后成熟 T 细胞免疫表型，通常可与其他成熟 T 细胞白血病相区别。
结论
尽管 T-PLL 的明确诊断需要采用整合临床资料、形态学、免疫表型、细胞遗传学/荧光原位杂交和分子特征的系统方法，但我们的结果表明，多参数流式细胞术的免疫表型分析极大地有助于诊断，并有助于这种成熟 T 细胞白血病的分类。
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