Statuto Teodora, D'Auria Fiorella, Del Vecchio Luigi, Mansueto Giovanna Rosaria, Villani Oreste, Lalinga Anna Vittoria, Possidente Luciana, Nozza Filomena, Vona Gabriella, Rago Luciana, Storto Giovanni, Gasparini Vanessa Rebecca, Zambello Renato, D'Arena Giovanni, Valvano Luciana
Laboratory of Clinical Research and Advanced Diagnostics, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy.
Unit of Clinical Pathology, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy.
Onco Targets Ther. 2020 Aug 3;13:7605-7614. doi: 10.2147/OTT.S258512. eCollection 2020.
Lymphoproliferative disorders are a heterogeneous group of malignant clonal proliferations of lymphocytes whose diagnosis remains challenging, despite diagnostic criteria are now well established, due to their heterogeneity in clinical presentation and immunophenotypic profile. Lymphoid T-cell disorders are more rarely seen than B-cell entities and more difficult to diagnose for the absence of a specific immunophenotypic signature. Flow cytometry is a useful tool in diagnosing T-cell lymphoproliferative disorders since it is not only able to better characterize T-cell neoplasms but also to resolve some very complicated cases, in particular those in which a small size population of neoplastic cells is available for the analysis. Here, we report three patients with mature T-cell neoplasms with atypical clinical and biological features in which analysis of peripheral blood and bone marrow specimens by means of multicolor flow cytometry was very useful to identify and characterize three rare T-cell lymphoproliferative disorders, such as angioimmunoblastic T-cell lymphoma, peripheral T-cell lymphoma not otherwise specified and T-cell prolymphocytic leukemia. The aim of this case series report is not only to describe three rare cases of lymphoproliferative neoplasms but also to raise awareness that a fast, highly sensitive, and reproducible procedure, such as flow cytometry immunophenotyping, can have a determinant diagnostic role in these patients.
淋巴增殖性疾病是一组异质性的淋巴细胞恶性克隆增殖性疾病，尽管目前诊断标准已很明确，但由于其临床表现和免疫表型特征的异质性，其诊断仍然具有挑战性。淋巴样T细胞疾病比B细胞实体少见，且由于缺乏特异性免疫表型特征而更难诊断。流式细胞术是诊断T细胞淋巴增殖性疾病的有用工具，因为它不仅能够更好地表征T细胞肿瘤，还能解决一些非常复杂的病例，特别是那些有少量肿瘤细胞可供分析的病例。在此，我们报告3例具有非典型临床和生物学特征的成熟T细胞肿瘤患者，通过多色流式细胞术分析外周血和骨髓标本对于识别和表征3种罕见的T细胞淋巴增殖性疾病非常有用，如血管免疫母细胞性T细胞淋巴瘤、外周T细胞淋巴瘤，非特指型和T细胞幼淋巴细胞白血病。本病例系列报告的目的不仅是描述3例罕见的淋巴增殖性肿瘤病例，还旨在提高人们的认识，即一种快速、高度敏感且可重复的程序，如流式细胞术免疫表型分析，在这些患者中可发挥决定性的诊断作用。
