Moore R R, Burt C T, Roberts M F
Biochim Biophys Acta. 1985 Sep 30;846(3):394-7. doi: 10.1016/0167-4889(85)90011-4.
31P-NMR spectra of intact larvae and pupae of Drosophila melanogaster have been obtained at 109.3 MHz. A major resonance in these samples has been identified as tyrosine-O-phosphate. Its chemical shift reflects the hemolymph plasma pH. Upon disruption of the organisms (necessary for chemical analyses of tyrosine-O-phosphate), phosphatases rapidly hydrolyze this phosphate ester, generating inorganic phosphate and free tyrosine.
已在109.3兆赫下获得黑腹果蝇完整幼虫和蛹的31P-核磁共振谱。这些样品中的一个主要共振峰已被鉴定为磷酸酪氨酸。其化学位移反映了血淋巴血浆的pH值。在破坏生物体(对磷酸酪氨酸进行化学分析所必需的)后,磷酸酶迅速水解这种磷酸酯,生成无机磷酸盐和游离酪氨酸。