完整酵母细胞的高分辨率31P核磁共振研究。
High resolution 31P nuclear magnetic resonance studies of intact yeast cells.
作者信息
Salhany J M, Yamane T, Shulman R G, Ogawa S
出版信息
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4966-70. doi: 10.1073/pnas.72.12.4966.
Abstract
High resolution 31P nuclear magnetic resonance (NMR) spectra at 145.7 MHZ are presented for intact yeast cells. Several peaks are resolved and assigned. They include the middle phosphate peaks from long chain or cyclic polyphosphates. Our results are consistent with the suggestion that these polyphosphates act as a phosphate store in the cell. We have also been able to measure cytoplasmic pH using the orthophosphate peak inside the cell, as compared with outside the cell. The results show that yeast cells maintain their cytoplasmic pH around 6.3. This value is considerably higher than the acidic extracellular pH at which they normally live. These preliminary results indicate that 31P NMR at 145.7 MHZ can be a rapid, informative, and non-invasive method for probing biochemical events within living cells.
摘要
给出了完整酵母细胞在145.7兆赫兹下的高分辨率31P核磁共振（NMR）谱。分辨并确定了几个峰。它们包括长链或环状多磷酸盐的中间磷酸盐峰。我们的结果与这些多磷酸盐在细胞中作为磷酸盐储存库的观点一致。与细胞外相比，我们还能够利用细胞内的正磷酸盐峰来测量细胞质pH值。结果表明，酵母细胞将其细胞质pH值维持在6.3左右。该值远高于它们正常生存的酸性细胞外pH值。这些初步结果表明，145.7兆赫兹的31P NMR可以成为一种用于探测活细胞内生化事件的快速、信息丰富且非侵入性的方法。
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本文引用的文献
1
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J Gen Physiol. 1963 May;46(5):1075-85. doi: 10.1085/jgp.46.5.1075.
2
Inorganic polyphosphates in biology: structure, metabolism, and function.生物学中的无机多聚磷酸盐：结构、代谢与功能
Bacteriol Rev. 1966 Dec;30(4):772-94. doi: 10.1128/br.30.4.772-794.1966.
3
Determination of intracellular pH by 31P magnetic resonance.通过31P磁共振测定细胞内pH值。
J Biol Chem. 1973 Oct 25;248(20):7276-8.
4
Observation of tissue metabolites using 31P nuclear magnetic resonance.使用31P核磁共振观察组织代谢物
Nature. 1974 Nov 22;252(5481):285-7. doi: 10.1038/252285a0.
5
Phosphate metabolism in intact human erythrocytes: determination by phosphorus-31 nuclear magnetic resonance spectroscopy.完整人体红细胞中的磷酸盐代谢：通过磷-31核磁共振光谱法测定
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2487-90. doi: 10.1073/pnas.71.6.2487.
6
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Proc Natl Acad Sci U S A. 1975 Sep;72(9):3482-5. doi: 10.1073/pnas.72.9.3482.
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