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味觉传感系统在虾加工副产物酶解产物特性表征中的应用。

Application of taste sensing system for characterisation of enzymatic hydrolysates from shrimp processing by-products.

作者信息

Cheung Imelda W Y, Li-Chan Eunice C Y

机构信息

The University of British Columbia, Faculty of Land and Food Systems, Food Nutrition and Health Program, 2205 East Mall, Vancouver, British Columbia V6T 1Z4, Canada.

出版信息

Food Chem. 2014 Feb 15;145:1076-85. doi: 10.1016/j.foodchem.2013.09.004. Epub 2013 Sep 11.

Abstract

The objective of this study was to investigate the potential of an instrumental taste-sensing system to distinguish between shrimp processing by-products hydrolysates produced using different proteases and hydrolysis conditions, and the possible association of taste sensor outputs with human gustatory assessment, salt content, and bioactivity. Principal component analysis of taste sensor output data categorised samples according to the proteases used for hydrolysis. High umami sensor outputs were characteristic of bromelain- and Flavourzyme-produced hydrolysates, compared to low saltiness and high bitterness outputs of Alcalase-produced hydrolysates, and high saltiness and low umami outputs of Protamex-produced hydrolysates. Extensively hydrolysed samples showed higher sourness outputs. Saltiness sensor outputs were correlated with conductivity and sodium content, while umami sensor responses were related to gustatory sweetness, bitterness and umami, as well as angiotensin-I converting enzyme inhibitory activity. Further research should explore the dose dependence and sensitivity of each taste sensor to specific amino acids and peptides.

摘要

本研究的目的是探究一种仪器化味觉传感系统区分使用不同蛋白酶和水解条件生产的虾加工副产物水解物的潜力,以及味觉传感器输出与人类味觉评估、盐含量和生物活性之间的可能关联。根据用于水解的蛋白酶,对味觉传感器输出数据进行主成分分析,对样品进行分类。与碱性蛋白酶生产的水解物低盐度和高苦味输出以及Protamex生产的水解物高盐度和低鲜味输出相比,菠萝蛋白酶和风味酶生产的水解物具有高鲜味传感器输出的特征。深度水解的样品显示出更高的酸味输出。盐度传感器输出与电导率和钠含量相关,而鲜味传感器响应与味觉甜度、苦味和鲜味以及血管紧张素-I转换酶抑制活性有关。进一步的研究应探索每个味觉传感器对特定氨基酸和肽的剂量依赖性和敏感性。

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