恶性疟原虫配子体上两个明显不重复的表位是传播阻断抗体的作用靶点。
Two apparently nonrepeated epitopes on gametes of Plasmodium falciparum are targets of transmission-blocking antibodies.
作者信息
Carter R, Bushell G, Saul A, Graves P M, Kidson C
出版信息
Infect Immun. 1985 Oct;50(1):102-6. doi: 10.1128/iai.50.1.102-106.1985.
Abstract
One-site and two-site immunoradiometric assays have been developed against an antigen on gametocytes of Plasmodium falciparum, using monoclonal antibodies (Mabs) which block transmission of the parasites to mosquitoes. Three such Mabs have been studied, each of which immunoprecipitates a complex of three gamete surface proteins of apparent Mr 260,000, 59,000, and 53,000 from Triton X-100 extracts of the parasites. The assays showed that the Mabs recognized one or the other of two distinct, nonrepeated epitopes on the target antigen(s). In the one-site assay certain combinations of two Mabs interacted at appropriate concentrations to enhance binding of the Mabs to the antigen. The same combinations of Mabs synergize to suppress infectivity of gametocytes to mosquitoes.
摘要
已利用可阻断疟原虫向蚊子传播的单克隆抗体（Mab），针对恶性疟原虫配子体上的一种抗原开发了单位点和双位点免疫放射分析方法。已研究了三种此类单克隆抗体，每种抗体都能从寄生虫的 Triton X - 100 提取物中免疫沉淀出三种表观分子量分别为 260,000、59,000 和 53,000 的配子表面蛋白复合物。分析表明，这些单克隆抗体识别靶抗原上两个不同的、非重复的表位中的一个或另一个。在单位点分析中，两种单克隆抗体的某些组合在适当浓度下相互作用，以增强单克隆抗体与抗原的结合。相同的单克隆抗体组合协同作用以抑制配子体对蚊子的感染性。
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本文引用的文献
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