Kosaka T, Kosaka K, Tateishi K, Hamaoka Y, Yanaihara N, Wu J Y, Hama K
J Comp Neurol. 1985 Sep 22;239(4):420-30. doi: 10.1002/cne.902390408.
The coexistence of cholecystokinin-octapeptide-like (CCK-L) and/or vasoactive-intestinal-polypeptide-like immunoreactive (VIP-LI) materials and glutamate decarboxylase (GAD) was studied in the rat hippocampus and dentate gyrus by means of immunohistochemistry. Consecutive 40-micron-thick sections were incubated in different antisera and those cells which were bisected by the plane of sectioning so as to be included at the paired surfaces of two adjacent sections were identified. The coexistence of the immunoreactivities for these peptides and GAD in the same cell could thus be determined by observing the immunoreactivity of the two halves of the cell, incubated in two different antisera. Almost all of the CCK-LI neurons were also GAD immunoreactive, whereas only about 10% of the GAD-immunoreactive neurons were CCK-LI. The percentages of GAD-immunoreactive neurons which were also immunoreactive for CCK were dependent on the laminar area in which they were found: i.e., 15-20% in the stratum radiatum of the hippocampus, about 10% in the stratum pyramidale, and about 6% in the stratum oriens. In contrast to the CCK-LI neurons, only about 40% of the VIP-LI neurons were identified to be also GAD immunoreactive, which might correspond to only part of the GAD-immunoreactive neurons. Furthermore the coexistence of VIP-LI and CCK-LI materials was recognized in about 10% of the CCK-LI neurons or about 35% of the VIP-LI neurons, indicating that some GABAergic neurons (presumably about 1%) in the rat hippocampus and dentate gyrus may contain both CCK-LI and VIP-LI materials.
采用免疫组织化学方法，研究了大鼠海马和齿状回中胆囊收缩素八肽样（CCK-L）和/或血管活性肠多肽样免疫反应物质（VIP-LI）与谷氨酸脱羧酶（GAD）的共存情况。将连续的40微米厚切片置于不同抗血清中孵育，识别那些被切片平面平分从而包含在两个相邻切片配对表面的细胞。通过观察同一细胞在两种不同抗血清中孵育后的两半细胞的免疫反应性，可确定这些肽与GAD在同一细胞中的共存情况。几乎所有CCK-LI神经元也呈GAD免疫反应性，而仅约10%的GAD免疫反应性神经元为CCK-LI。对CCK也呈免疫反应性的GAD免疫反应性神经元的百分比取决于它们所在的层区：即海马辐射层中为15 - 20%，锥体细胞层中约为10%，原层中约为6%。与CCK-LI神经元不同，仅约40%的VIP-LI神经元被确定也呈GAD免疫反应性，这可能仅对应部分GAD免疫反应性神经元。此外，在约10%的CCK-LI神经元或约35%的VIP-LI神经元中发现了VIP-LI和CCK-LI物质的共存，表明大鼠海马和齿状回中的一些GABA能神经元（可能约1%）可能同时含有CCK-LI和VIP-LI物质。
