Kosaka T, Tauchi M, Dahl J L
National Institute for Physiological Sciences, Okazaki, Japan.
Exp Brain Res. 1988;70(3):605-17. doi: 10.1007/BF00247609.
The coexistence of immunoreactivities for choline acetyltransferase (ChAT) and glutamic acid decarboxylase (GAD) and/or gamma-aminobutyric acid (GABA) was revealed in some brain regions of the rat, using the peroxidase-antiperoxidase method. Consecutive 40 micron thick vibratome sections were incubated in different antisera and those cells which were bisected by the plane of sectioning so as to be included at the paired surfaces of two adjacent sections were identified. The coexistence of the immunoreactivities for ChAT and GAD or GABA in the same cell could thus be determined by observing the immunoreactivity of the two halves of the cell incubated in two different antisera. In the retina, cerebral cortex, basal forebrain and spinal cord, colocalization of ChAT-like and GAD-like or GABA-like immunoreactivities was observed in some cell types, whereas no such colocalization was observed in cells in the striatum or brainstem. In the retina, the majority of ChAT-like immunoreactive (ChAT-LI) amacrine cells contained GABA-like or GAD-like immunoreactivity. About half of the ChAT-LI neurons in the cerebral cortex showed GABA-like immunoreactivity. In the basal forebrain only a small proportion of ChAT-LI neurons (0.6%) contained GAD-like immunoreactivity. In the spinal cord, about one-third of ChAT-LI central canal cluster cells and about half of ChAT-LI dorsal horn cells showed GAD-like and/or GABA-like immunoreactivities. These observations indicate the possible coexistence of two classical transmitters, GABA and acetylcholine, in various brain regions and spinal cord of the rat.
采用过氧化物酶抗过氧化物酶法，在大鼠的一些脑区中发现了胆碱乙酰转移酶（ChAT）与谷氨酸脱羧酶（GAD）和/或γ-氨基丁酸（GABA）免疫反应性的共存情况。将连续的40微米厚的振动切片机切片置于不同的抗血清中孵育，识别那些被切片平面一分为二从而包含在两个相邻切片的配对表面上的细胞。通过观察在两种不同抗血清中孵育的细胞两半的免疫反应性，就可以确定同一细胞中ChAT和GAD或GABA免疫反应性的共存情况。在视网膜、大脑皮层、基底前脑和脊髓中，在一些细胞类型中观察到了ChAT样和GAD样或GABA样免疫反应性的共定位，而在纹状体或脑干的细胞中未观察到这种共定位。在视网膜中，大多数ChAT样免疫反应性（ChAT-LI）无长突细胞含有GABA样或GAD样免疫反应性。大脑皮层中约一半的ChAT-LI神经元显示出GABA样免疫反应性。在基底前脑中，只有一小部分ChAT-LI神经元（0.6%）含有GAD样免疫反应性。在脊髓中，约三分之一的ChAT-LI中央管簇细胞和约一半的ChAT-LI背角细胞显示出GAD样和/或GABA样免疫反应性。这些观察结果表明，在大鼠的各种脑区和脊髓中，两种经典递质GABA和乙酰胆碱可能共存。
