van Sande M, Scharpé S L, Neels H M, Kasahara Y
J Clin Chem Clin Biochem. 1985 Jul;23(7):381-6. doi: 10.1515/cclm.1985.23.7.381.
Angiotensin-converting enzyme (EC 3.4.15.1) exhibits distinctive tissue differences in electrophoretic behaviour. Human tissue homogenates and biological fluids were subjected to agar gel and polyacrylamide gel electrophoresis. The gels were cut into slices and enzymatic activity detected by a specific colorimetric reaction. The technique allows localization of the enzyme in the electrophoretogram and gives an estimate of the total enzymatic activity of a tissue or biological fluid. Several tissues were found to show multiple forms of angiotensin-converting enzyme. The specificity of the assay was determined by running the samples after the addition of captopril and by adding proteinase inhibitors to the incubation mixture.
血管紧张素转换酶（EC 3.4.15.1）在电泳行为上表现出独特的组织差异。对人体组织匀浆和生物体液进行琼脂糖凝胶电泳和聚丙烯酰胺凝胶电泳。将凝胶切成薄片，通过特定的比色反应检测酶活性。该技术可在电泳图谱中定位酶，并估算组织或生物体液中的总酶活性。发现几种组织呈现多种形式的血管紧张素转换酶。通过在加入卡托普利后运行样品以及向孵育混合物中添加蛋白酶抑制剂来确定测定的特异性。
