人泌尿生殖道组织中血管紧张素转换酶的三肽基羧肽酶活性。

Tripeptidyl carboxypeptidase activity of angiotensin-converting enzyme in human tissues of the urogenital tract.

作者信息

van Sande M, Inokuchi J, Nagamatsu A, Scharpé S, Neels H, Van Camp K

出版信息

Urol Int. 1985;40(2):100-2. doi: 10.1159/000281046.

Abstract

The tripeptidyl carboxypeptidase activity of angiotensin-converting enzyme (EC 3.4.15.1) has been determined in several human tissues of the urogenital tract. Benzoyl-glycyl-L-seryl-L-prolyl-L-phenylalanine was used as substrate. The cleaved benzoyl-glycine was measured by means of high-performance liquid chromatography. Results obtained showed a high enzymatic activity for seminal plasma, vesicula seminalis and benign prostatic hyperplasia. Normal prostate and in particular prostatic adenocarcinoma have low enzymatic activity. The activity of the enzyme is sensitive to inhibition by the angiotensin-converting enzyme inhibitor captopril.

摘要

已在泌尿生殖道的多种人体组织中测定了血管紧张素转换酶（EC 3.4.15.1）的三肽基羧肽酶活性。使用苯甲酰 - 甘氨酰 - L - 丝氨酰 - L - 脯氨酰 - L - 苯丙氨酸作为底物。通过高效液相色谱法测定裂解产生的苯甲酰甘氨酸。所得结果显示精浆、精囊和良性前列腺增生具有较高的酶活性。正常前列腺，尤其是前列腺腺癌的酶活性较低。该酶的活性对血管紧张素转换酶抑制剂卡托普利的抑制作用敏感。