在高pH值下配体与亚铁细胞色素c的结合
Ligand binding to ferrocytochrome c at high pH.
作者信息
Moore T A, Greenwood C, Wilson M T
出版信息
Biochem J. 1975 May;147(2):335-41. doi: 10.1042/bj1470335.
Abstract
Ferrocytochrome c has been shown to bind two molecules of CO at pH 14. The second CO is thought to be bound only when the cytochrome c molecule is denatured, and once bound appears to be spectrally silent. Insolubilization of native cytochrome c prevents the binding of the second CO molecule. A scheme is proposed to explain these observations based on evidence from static titrations and flash-photolysis experiments, use of carboxymethyl cytochrome c and insoluble cytochrome c, and use of cyanide instead of CO as a ligand.
摘要
亚铁细胞色素c已被证明在pH 14时能结合两个一氧化碳分子。据认为，第二个一氧化碳分子只有在细胞色素c分子变性时才会结合，并且一旦结合，在光谱上似乎是无信号的。天然细胞色素c的不溶性可阻止第二个一氧化碳分子的结合。基于静态滴定和闪光光解实验的证据、羧甲基细胞色素c和不溶性细胞色素c的使用，以及使用氰化物代替一氧化碳作为配体，提出了一个方案来解释这些观察结果。
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