Holzwarth M A, Brownfield M S
Neuroendocrinology. 1985 Sep;41(3):230-6. doi: 10.1159/000124182.
Immunoreactive serotonin is demonstrated to be present in 75% of rat adrenal medullary cells using an antibody to serotonin and peroxidase-antiperoxidase immunocytochemical method. The concentration of serotonin in rat adrenals was found to be 7.7 +/- 0.1 X 10(-6) mol/kg wet weight by high-performance liquid chromatography with electrochemical detection. Drugs that block serotonin synthesis (p-chlorophenylalanine) or deplete biogenic amines (reserpine) diminish immunostaining. The serotonin precursor L-tryptophan and pargyline, a monoamine oxidase inhibitor, augment staining in reserpine-depleted adrenals. Serotonin is localized in those medullary cells which contain phenylethanolamine-N-methyltransferase, an enzyme which is necessary for the synthesis of epinephrine. We conclude, therefore, that serotonin coexists with epinephrine in rat adrenal medullary chromaffin cells. These results suggest that the adrenal medulla may play a major role in the metabolism of serotonin.
采用抗血清素抗体和过氧化物酶 - 抗过氧化物酶免疫细胞化学方法，证明75%的大鼠肾上腺髓质细胞中存在免疫反应性血清素。通过高效液相色谱电化学检测发现，大鼠肾上腺中血清素的浓度为7.7±0.1×10⁻⁶摩尔/千克湿重。阻断血清素合成的药物（对氯苯丙氨酸）或消耗生物胺的药物（利血平）会减少免疫染色。血清素前体L - 色氨酸和单胺氧化酶抑制剂帕吉林可增强利血平处理后肾上腺的染色。血清素定位于那些含有苯乙醇胺 - N - 甲基转移酶的髓质细胞中，该酶是肾上腺素合成所必需的。因此，我们得出结论，血清素与肾上腺素共存于大鼠肾上腺髓质嗜铬细胞中。这些结果表明，肾上腺髓质可能在血清素的代谢中起主要作用。
