Ritchie P K, Knight H H, Ashby M, Judd A M
Department of Zoology, Brigham Young University, 585 WIDB, 84602, Provo, UT.
Endocrine. 1996 Dec;5(3):291-7. doi: 10.1007/BF02739062.
Interleukin-6 (IL-6) and tumor necrosis factor (TNF) are secreted by rat adrenal zona glomerulosa cells. Serotonin increases the release of aldosterone, corti-costerone, and cortisol from the adrenal cortex. Therefore, the effects of serotonin on IL-6 and TNF release from rat adrenal zona glomerulosa cells were investigated. Cultures of rat adrenal zona glomerulosa cells were enzymatically prepared and cultured for 4-6 d. The cells were then exposed to serum-free RPMl-1640 medium containing vehicle (RPMl medium alone), serotonin, and/or endotoxin, interleukin-1β, or adrenocorticotrophic hormone (ACTH). Following a 5-h incubation, medium was removed from the cells, and IL-6 and TNF content of this medium determined with bioassays. Serotonin (1-1000 nM) increased basal IL-6 release from zona glomerulosa cells, but inhibited basal TNF release from these cells. Endotoxin and interleukin-1β (IL-1β) increased IL-6 and TNF release from zona glomerulosa cells. Serotonin potentiated IL-6 release stimulated by endotoxin and IL-1β, but inhibited TNF release stimulated by these agents. Serotonin potentiated ACTH-stimulated IL-6 release. Serotonin had no effect on IL-6 release from rat anterior pituitary cells. Because IL-6, TNF, and serotonin modify the release of aldosterone and glucocorticoids from adrenal cells, the stimulatory effects of serotonin on aldosterone and glucocorticoid release may be mediated in part by the effects of serotonin on IL-6 and TNF release from adrenal cells.
白细胞介素-6（IL-6）和肿瘤坏死因子（TNF）由大鼠肾上腺球状带细胞分泌。血清素可增加肾上腺皮质醛固酮、皮质酮和皮质醇的释放。因此，研究了血清素对大鼠肾上腺球状带细胞释放IL-6和TNF的影响。采用酶解法制备大鼠肾上腺球状带细胞培养物，并培养4 - 6天。然后将细胞暴露于含有载体（仅RPMI培养基）、血清素和/或内毒素、白细胞介素-1β或促肾上腺皮质激素（ACTH）的无血清RPMI-1640培养基中。孵育5小时后，从细胞中取出培养基，用生物测定法测定该培养基中IL-6和TNF的含量。血清素（1 - 1000 nM）增加了球状带细胞基础IL-6的释放，但抑制了这些细胞基础TNF的释放。内毒素和白细胞介素-1β（IL-1β）增加了球状带细胞IL-6和TNF的释放。血清素增强了内毒素和IL-1β刺激的IL-6释放，但抑制了这些因子刺激的TNF释放。血清素增强了ACTH刺激的IL-6释放。血清素对大鼠垂体前叶细胞IL-6的释放没有影响。由于IL-6、TNF和血清素可改变肾上腺细胞醛固酮和糖皮质激素的释放，血清素对醛固酮和糖皮质激素释放的刺激作用可能部分是由血清素对肾上腺细胞IL-6和TNF释放的影响介导的。
