Yoshioka K, Michael A F, Velosa J, Fish A J
Am J Pathol. 1985 Oct;121(1):156-65.
The reactivity of 10 human anti-glomerular basement membrane (GBM) autoantibodies with basement membrane antigens of human adult and infant kidney, lung, placenta, and skin was examined by ELISA and immunofluorescence microscopy. All autoantibodies were previously shown to react with adult kidney by indirect immunofluorescence and with collagenase-digested adult GBM by ELISA. Four antibodies (group A) were positive on infant and fetal kidney sections by immunofluorescence, and six antibodies were negative (group B). By ELISA both groups of antibodies were reactive with collagenase digests of infant GBM. After denaturation with 6M urea (pH 3.5) infant and fetal kidney sections reacted with group B autoantibodies by immunofluorescence, which indicated that the antigen(s) was masked. Hidden antigenic determinants in lung, placenta, and skin were reactive with groups A and B autoantibodies only after acid urea denaturation of tissue sections. Within each group variability in reactivity of autoantibodies with basement membranes suggested further heterogeneity.
通过酶联免疫吸附测定（ELISA）和免疫荧光显微镜检查，检测了10种人抗肾小球基底膜（GBM）自身抗体与成人及婴儿肾脏、肺、胎盘和皮肤的基底膜抗原的反应性。所有自身抗体先前已通过间接免疫荧光显示与成人肾脏发生反应，并通过ELISA显示与胶原酶消化的成人GBM发生反应。四种抗体（A组）通过免疫荧光在婴儿和胎儿肾脏切片上呈阳性，六种抗体呈阴性（B组）。通过ELISA，两组抗体均与婴儿GBM的胶原酶消化产物发生反应。用6M尿素（pH 3.5）变性后，婴儿和胎儿肾脏切片通过免疫荧光与B组自身抗体发生反应，这表明抗原被掩盖。仅在组织切片经酸性尿素变性后，肺、胎盘和皮肤中隐藏的抗原决定簇才与A组和B组自身抗体发生反应。每组内自身抗体与基底膜反应性的变异性表明存在进一步的异质性。
