Wan Y J, Wu T C, Chung A E, Damjanov I
J Cell Biol. 1984 Mar;98(3):971-9. doi: 10.1083/jcb.98.3.971.
Two monoclonal antibodies raised against laminin isolated from a mouse parietal yolk sac cell line were used for immunohistochemical studies of basement membranes of the mouse embryo and various fetal and adult tissues. No immunoreactivity with either of the two monoclonal antibodies could be detected in the preimplantation-stage embryos, although it has been shown that these embryos contain extracellular laminin reactive with the conventional polyclonal antilaminin antibodies. Reichert's membrane in early postimplantation stages of development reacted with the monoclonal antibody LAM-I but not with the antibody LAM-II. However, from day 8 of pregnancy onward the Reichert's membrane reacted with both antibodies. Basement membranes of the embryo proper were unreactive with both monoclonal antibodies until day 12 of pregnancy. By day 14 some basement membranes of the fetal tissues became reactive with one or both monoclonal antibodies, whereas others remained still unreactive. In the 17-d fetus and the newborn mouse most of the basement membranes reacted with both monoclonal antibodies, whereas others still reacted with only one. Similar heterogeneity in the immunoreactivity of basement membranes of various tissues was noted in the adult mouse as well. These results indicate that the immunoreactivity of laminin in the extracellular matrix changes during development and that the basement membranes in various anatomic locations display heterogeneity even in the adult mouse.
两种针对从小鼠壁层卵黄囊细胞系分离出的层粘连蛋白产生的单克隆抗体，被用于对小鼠胚胎以及各种胎儿和成年组织的基底膜进行免疫组织化学研究。在植入前阶段的胚胎中，未检测到这两种单克隆抗体中的任何一种的免疫反应性，尽管已经表明这些胚胎含有与传统多克隆抗层粘连蛋白抗体反应的细胞外层粘连蛋白。植入后早期发育阶段的赖歇特氏膜与单克隆抗体LAM-I反应，但不与抗体LAM-II反应。然而，从妊娠第8天起，赖歇特氏膜与两种抗体都反应。直到妊娠第12天，胚胎本身的基底膜与两种单克隆抗体都无反应。到第14天，胎儿组织的一些基底膜与一种或两种单克隆抗体发生反应，而其他基底膜仍然无反应。在17天的胎儿和新生小鼠中，大多数基底膜与两种单克隆抗体都反应，而其他基底膜仍然只与一种抗体反应。在成年小鼠的各种组织的基底膜的免疫反应性中也观察到类似的异质性。这些结果表明，细胞外基质中层粘连蛋白的免疫反应性在发育过程中发生变化，并且即使在成年小鼠中，不同解剖位置的基底膜也表现出异质性。
