Tan Lin, Yu Jin-Tai, Liu Qiu-Yan, Tan Meng-Shan, Zhang Wei, Hu Nan, Wang Ying-Li, Sun Lei, Jiang Teng, Tan Lan
Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, PR China.
Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, PR China; Department of Neurology, Qingdao Municipal Hospital, College of Medicine and Pharmaceutics, Ocean University of China, Qingdao, PR China; Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing, PR China.
J Neurol Sci. 2014 Jan 15;336(1-2):52-6. doi: 10.1016/j.jns.2013.10.002. Epub 2013 Oct 9.
MicroRNAs (miRNAs) are endogenous small RNAs of 21-25 nucleotides that post-transcriptionally regulate gene expressions. Recently, circulating miRNAs have been reported as promising biomarkers for neurodegenerative disorders and processes affecting the central nervous system. This study was conducted to investigate the potential role of serum miRNAs as diagnostic biomarkers for Alzheimer's disease (AD).
Serum samples were obtained from 105 probable AD patients and 150 age- and gender-matched normal controls. The serum concentrations of miRNAs miR-9, miR-29a, miR-29b, miR-101, miR-125b, and miR-181c were measured with a real-time quantitative reverse transcriptase PCR (qRT-PCR) method.
We found both miR-125b and miR-181c were down-regulated while miR-9 was up-regulated in serum of AD patients compared with that of normal controls. Among the receiver operating characteristic (ROC) results, miR-125b alone showed its priority with a specificity up to 68.3% and a sensitivity of 80.8%. Importantly, miR-125b was correlated with the Mini Mental State Examination (MMSE) in AD patients.
Our results indicate that serum miR-125b may serve as a useful noninvasive biomarker for AD.
微小RNA（miRNA）是一类21 - 25个核苷酸的内源性小RNA，可在转录后水平调控基因表达。最近，循环miRNA已被报道为神经退行性疾病和影响中枢神经系统过程的有前景的生物标志物。本研究旨在探讨血清miRNA作为阿尔茨海默病（AD）诊断生物标志物的潜在作用。
从105例可能的AD患者和150例年龄及性别匹配的正常对照中获取血清样本。采用实时定量逆转录聚合酶链反应（qRT-PCR）方法检测血清中miR-9、miR-29a、miR-29b、miR-101、miR-125b和miR-181c的浓度。
我们发现与正常对照相比，AD患者血清中miR-125b和miR-181c均下调，而miR-9上调。在受试者工作特征（ROC）结果中，单独的miR-125b表现突出，特异性高达68.3%，敏感性为80.8%。重要的是，miR-125b与AD患者的简易精神状态检查（MMSE）相关。
我们的结果表明血清miR-125b可能作为AD有用的非侵入性生物标志物。
