Food & Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Laboratory of Food and Biomolecular Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
J Alzheimers Dis. 2014;39(2):253-9. doi: 10.3233/JAD-130932.
The development of Alzheimer's disease (AD) biomarkers remains an unmet challenge, and new approaches that can improve current AD biomarker strategies are needed. Recent reports suggested that microRNA (miRNA) profiling of biological fluids has emerged as a diagnostic tool for several pathologic conditions. In this study, we measured six candidate miRNAs (miR-9, miR-29a, miR-29b, miR-34a, miR-125b, and miR-146a) in plasma and cerebrospinal fluid (CSF) of AD and normal subjects by using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) to evaluate their potential usability as AD biomarkers. The qRT-PCR results showed that plasma miR-34a and miR-146a levels, and CSF miR-34a, miR-125b, and miR-146a levels in AD patients were significantly lower than in control subjects. On the other hand, CSF miR-29a and miR-29b levels were significantly higher than in control subjects. Our results provide a possibility that miRNAs detected in plasma and CSF can serve as biomarkers for AD.
阿尔茨海默病（AD）生物标志物的发展仍然是一个未满足的挑战，需要新的方法来改进当前的 AD 生物标志物策略。最近的报告表明，生物体液中的 microRNA（miRNA）谱分析已成为几种病理状况的诊断工具。在这项研究中，我们通过定量逆转录聚合酶链反应（qRT-PCR）测量了 AD 和正常受试者血浆和脑脊液（CSF）中的六种候选 miRNA（miR-9、miR-29a、miR-29b、miR-34a、miR-125b 和 miR-146a），以评估它们作为 AD 生物标志物的潜在可用性。qRT-PCR 结果表明，AD 患者的血浆 miR-34a 和 miR-146a 水平以及 CSF miR-34a、miR-125b 和 miR-146a 水平明显低于对照组。另一方面，CSF miR-29a 和 miR-29b 水平明显高于对照组。我们的结果提供了一种可能性，即血浆和 CSF 中检测到的 miRNAs 可以作为 AD 的生物标志物。
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