Salgado Maristela Marques, Gonçalves Maria Gisele, Fukasawa Lucila Okuyama, Higa Fábio Takenori, Paulino Juliana Thalita, Sacchi Cláudio Tavares
Arq Neuropsiquiatr. 2013 Sep;71(9B):672-6. doi: 10.1590/0004-282X20130148.
Bacterial meningitis (BM) is a severe disease and still represents a serious public health problem with high rates of morbidity and mortality. The most common cases of BM around the world, mainly in Brazil, have been caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae type b. Bacterial culture is the gold-standard technique for BM confirmation, but approximately 50% of suspected cases are not culture-confirmed, due to problems related to improper transportation and seeding or previous antibiotic treatment. Immunological methods present low sensitivity and have possibility of cross-reactions. Real time PCR (qPCR) is a molecular technique and has been successful used for BM diagnosis at Instituto Adolfo Lutz in São Paulo State, Brazil, since 2007. The incorporation of qPCR in the Public Health surveillance routine in our state resulted in diminishing 50% of undetermined BM cases. Our efforts are focused on qPCR implementation in the BM diagnostic routine throughout Brazil.
细菌性脑膜炎（BM）是一种严重疾病，仍然是一个严重的公共卫生问题，发病率和死亡率很高。全球BM最常见的病例主要在巴西，由脑膜炎奈瑟菌、肺炎链球菌和b型流感嗜血杆菌引起。细菌培养是确诊BM的金标准技术，但由于运输和接种不当或先前接受过抗生素治疗等问题，约50%的疑似病例未通过培养确诊。免疫方法灵敏度低且有交叉反应的可能性。实时荧光定量聚合酶链反应（qPCR）是一种分子技术，自2007年以来已成功用于巴西圣保罗州阿道夫·卢茨研究所的BM诊断。在本州公共卫生监测常规工作中采用qPCR，使未确诊的BM病例减少了50%。我们的工作重点是在巴西全国的BM诊断常规工作中实施qPCR。
