四膜虫中钙离子依赖性钾电流降低的突变体的遗传分析。
Genetic analysis of mutants with a reduced Ca2+-dependent K+ current in Paramecium tetraurelia.
作者信息
Hinrichsen R D, Amberger E, Saimi Y, Burgess-Cassler A, Kung C
出版信息
Genetics. 1985 Nov;111(3):433-45. doi: 10.1093/genetics/111.3.433.
Abstract
Two mutants of Paramecium tetraurelia with greatly reduced Ca2+-dependent K+ currents have been isolated and genetically analyzed. These mutants, designated pantophobiac, give much stronger behavioral responses to all stimuli than do wild-type cells. Under voltage clamp, the Ca2+-dependent K+ current is almost completely eliminated in these mutants, whereas the Ca2+ current is normal. The two mutants, pntA and pntB, are recessive and unlinked to each other. pntA is not allelic to several other ion-channel mutants of P. tetraurelia. The microinjection of a high-speed supernatant fraction of wild-type cytoplasm into either pantophobiac mutant caused a temporary restoration to the wild-type phenotype.
摘要
已分离并对两种依赖钙离子的钾离子电流大幅降低的四膜虫突变体进行了遗传分析。这些被命名为泛恐惧症的突变体，对所有刺激的行为反应比野生型细胞强烈得多。在电压钳制下，这些突变体中依赖钙离子的钾离子电流几乎完全消除，而钙离子电流正常。这两个突变体，pntA和pntB，是隐性的且彼此不连锁。pntA与四膜虫的其他几个离子通道突变体不等位。将野生型细胞质的高速上清液组分显微注射到任一泛恐惧症突变体中，都会导致其暂时恢复为野生型表型。
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