对镁反应存在缺陷的草履虫突变体的分离与鉴定
Isolation and characterization of paramecium mutants defective in their response to magnesium.
作者信息
Preston R R, Kung C
机构信息
Laboratory of Molecular Biology, University of Wisconsin-Madison 53706.
出版信息
Genetics. 1994 Jul;137(3):759-69. doi: 10.1093/genetics/137.3.759.
Abstract
Four mutant strains of Paramecium tetraurelia with a reduced ability to respond behaviorally to Mg2+ have been isolated. Voltage-clamp analyses showed that their Mg2+ insensitivity is associated with a reduced Ca(2+)-dependent Mg2+ current. The four mutants, which have been doubled "eccentric," result from recessive mutations in two unlinked loci, xntA and xntB. Further analysis of xntA1 showed it to be unlinked to any of the behavioral mutants of P. tetraurelia described previously, but it is allelic to d4-521, a "K(+)-resistant" strain, and d4-596, a "Ba(2+)-shy" mutant. The varied pleiotropic effects of xntA1, which include increased resistance to Ni2+ and Zn2+ poisoning, suggest that the locus encodes a central regulator of cell function in Paramecium.
摘要
已分离出四株对Mg2+行为反应能力降低的四膜虫突变株。电压钳分析表明，它们对Mg2+的不敏感性与Ca(2+)依赖性Mg2+电流的降低有关。这四个已加倍的“偏心”突变体是由两个不连锁基因座xntA和xntB中的隐性突变产生的。对xntA1的进一步分析表明，它与先前描述的四膜虫的任何行为突变体都不连锁，但它与“抗K(+)”菌株d4-521和“怕Ba(2+)”突变体d4-596等位。xntA1具有多种多效性效应，包括对Ni2+和Zn2+中毒的抗性增加，这表明该基因座编码四膜虫细胞功能的中央调节因子。
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