The aim of this paper was to determine for first time the influence of the main meteorological parameters on the atmospheric fungal spore concentration in Havana (Cuba). This city is characterized by a subtropical climate with two different marked annual rainfall seasons during the year: a "dry season" and a "rainy season". A nonviable volumetric methodology (Lanzoni VPPS-2000 sampler) was used to sample airborne spores. The total number of spores counted during the 2 years of study was 293,594, belonging to 30 different genera and five spore types. Relative humidity was the meteorological parameter most influencing the atmospheric concentration of the spores, mainly during the rainy season of the year. Winds coming from the SW direction also increased the spore concentration in the air. In terms of spore intradiurnal variation we found three different patterns: morning maximum values for Cladosporium, night peaks for Coprinus and Leptosphaeria, and uniform behavior throughout the whole day for Aspergillus/Penicillium."