溃疡性结肠炎相关结直肠癌差异表达 microRNA 的筛选。
Screening of differentially expressed microRNA in ulcerative colitis related colorectal cancer.
机构信息
Department of Gastroenterology, The Second People's Hospital of Shenzhen (The First Affiliated Hospital of Shenzhen University), Shenzhen 518035, China.
出版信息
Asian Pac J Trop Med. 2013 Dec;6(12):972-6. doi: 10.1016/S1995-7645(13)60174-1.
OBJECTIVE
To investigate the differential expression of microRNA (miRNA) in colon between ulcerative colitis (UC) and ulcerative colitis related colorectal cancer (UCRCC).
METHODS
An UC mouse model was built by dextran sodium sulfate, and an UCRCC mouse model by dextran sodium sulfate and 1,2-diformylhydrazine. RNAs were extracted from the colon, purified and hybridized with fluorescence-labeled miRNA oligonucleotide gene chip. Real-time fluorescence quantitative PCR was used to verify the expression variation of miRNA. SAM was employed for the data analysis.
RESULTS
The up-regulated miRNAs in colon cancer included has-miR-194, has-miR-215, has-miR-93, has-miR-192, has-miR-92a, has-miR-29b, and has-miR-20a (median false discovery rate<5%), while the down-regulated miRNAs were has-miR-1231, has-miR-195, has-miR-143, and has-miR-145 (median false discovery rate<5%).
CONCLUSIONS
Significant differential expression of miRNA was found between the UC mouse and UCRCC mouse, which may be related to the onset, erosion and transfer of colorectal cancer.
目的
研究溃疡性结肠炎（UC）和溃疡性结肠炎相关结直肠癌（UCRCC）结肠组织中微小 RNA（miRNA）的差异表达。
方法
采用葡聚糖硫酸钠诱导 UC 小鼠模型，1,2-二氟二肼诱导 UCRCC 小鼠模型。提取结肠组织 RNA，用荧光标记 miRNA 寡核苷酸基因芯片杂交，实时荧光定量 PCR 验证 miRNA 的表达变化。采用 SAM 进行数据分析。
结果
结肠癌中上调的 miRNA 包括 has-miR-194、has-miR-215、has-miR-93、has-miR-192、has-miR-92a、has-miR-29b 和 has-miR-20a（中位数假发现率<5%），而下调的 miRNA 有 has-miR-1231、has-miR-195、has-miR-143 和 has-miR-145（中位数假发现率<5%）。
结论
UC 小鼠和 UCRCC 小鼠之间存在显著差异表达的 miRNA，可能与大肠癌的发生、侵蚀和转移有关。
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