Swati Valmiki, Jaishree Paul, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
World J Gastroenterol. 2017 Aug 7;23(29):5324-5332. doi: 10.3748/wjg.v23.i29.5324.
To investigate the miRNA expression in colonic mucosal biopsies from endoscopically inflamed and non inflamed regions of ulcerative colitis (UC) patients.
Colonic mucosal pinch biopsies were analyzed from the inflamed and non inflamed regions of same UC patient. Total RNA was isolated and differential miRNA profiling was done using microarray platform. Quantitative Real Time PCR was performed in colonic biopsies from inflamed ( = 8) and non-inflamed ( = 8) regions of UC and controls ( = 8) to validate the differential expression of miRNA. Potential targets of dysregulated miRNA were identified by using prediction tools and probable role of these miRNA in inflammatory pathways were predicted.
The miRNA profile of inflamed colonic mucosa differs significantly from the non-inflamed. Real time PCR analysis showed that some of the miRNA were differentially expressed in the inflamed mucosa as compared to non inflamed mucosa and controls (miR-125b, miR-223, miR-138, and miR-155), while (miR-200a) did not show any significant changes. In contrast to microarray, where miR-378d showed downregulation in the inflamed mucosa, qRT-PCR showed a significant upregulation in the inflamed mucosa as compared to the non inflamed. The prediction analysis revealed that the genes targeted by these miRNAs play role in the major signaling pathways like MAPK pathway, NF-κB signaling pathway, cell adhesion molecules which are all assciated with UC.
The present study reports disease specific alteration in the expression of miR-125b, miR-155, miR-223 and miR-138 in UC patients and also predict their biological significance.
研究溃疡性结肠炎（UC）患者内镜下炎症和非炎症区域结肠黏膜活检中的 miRNA 表达。
对同一 UC 患者的炎症和非炎症区域的结肠黏膜钳取活检进行分析。分离总 RNA，采用微阵列平台进行差异 miRNA 谱分析。对 UC 患者炎症（=8）和非炎症（=8）区域以及对照（=8）的结肠活检进行定量实时 PCR，以验证 miRNA 的差异表达。使用预测工具鉴定失调 miRNA 的潜在靶标，并预测这些 miRNA 在炎症途径中的可能作用。
炎症结肠黏膜的 miRNA 谱与非炎症黏膜有显著差异。实时 PCR 分析显示，与非炎症黏膜和对照相比，一些 miRNA 在炎症黏膜中表达差异（miR-125b、miR-223、miR-138 和 miR-155），而（miR-200a）则没有显示任何显著变化。与微阵列相比，miR-378d 在炎症黏膜中显示下调，实时 PCR 显示与非炎症黏膜相比，炎症黏膜中显著上调。预测分析表明，这些 miRNA 靶向的基因在主要信号通路中发挥作用，如 MAPK 通路、NF-κB 信号通路、细胞黏附分子，这些都与 UC 相关。
本研究报告了 UC 患者中 miR-125b、miR-155、miR-223 和 miR-138 的表达出现疾病特异性改变，并预测了它们的生物学意义。
