Kim Se Woong, Shrestha Saroj Kumar, Chuluunbaatar Badmaarag-Altai, Soh Yunjo
Laboratory of Pharmacology, School of Pharmacy, Jeonbuk National University, Jeonju 54896, Republic of Korea.
Jeonbuk National University Hospital, Jeonju 54896, Republic of Korea.
Biomol Ther (Seoul). 2025 Jan 1;33(1):203-209. doi: 10.4062/biomolther.2024.104. Epub 2024 Dec 5.
The active component in cannabis, cannabidiol (CBD), was first isolated from the hemp plant in 1940. Chronic pain, inflammation, migraines, depression, and anxiety have long been treated with CBD. The fundamental mechanisms of CBD's effects on periodontal inflammation have yet to be fully understood. The amino sulfonic acid taurine is a substance that naturally exists in the body and is an inhibitory modulator of inflammation. This study examined the effects of CBD, taurine, and their combination on inflammatory cytokines and periodontitis . To assess the expression of inflammatory markers of iNOS, COX-2, TNF-α, and IL-1β, as well as TRAP count and resorbed pit areas, CBD and taurine were applied to RAW264.7 cells. The following groups of 45 Sprague-Dawley rats each were created: control (healthy), vehicle (induced periodontitis), low- and high-dose-CBD with taurine which were each treated for an additional 21 days. Rat teeth were obtained and subjected to histomorphometric studies. The combination of the two significantly decreased the expression of inflammatory markers TNF-α and IL-1β and the amount of TRAP+ cells and resorbed pit areas. Among rats with -induced periodontitis, the alveolar bone resorption levels, periodontal pocket depth, and distance between cementoenamel junction (CEJ) and alveolar bone crest (ABC) were significantly reduced after treatment with CBD and taurine, suggesting that combining CBD with taurine could be a novel therapeutic agent against periodontal disease.
大麻中的活性成分大麻二酚（CBD）于1940年首次从大麻植物中分离出来。长期以来，慢性疼痛、炎症、偏头痛、抑郁症和焦虑症都用CBD进行治疗。CBD对牙周炎影响的基本机制尚未完全了解。氨基磺酸牛磺酸是一种天然存在于体内的物质，是炎症的抑制调节剂。本研究考察了CBD、牛磺酸及其组合对炎性细胞因子和牙周炎的影响。为了评估诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）等炎症标志物的表达，以及抗酒石酸酸性磷酸酶（TRAP）计数和吸收凹陷面积，将CBD和牛磺酸应用于RAW264.7细胞。创建了以下几组，每组45只斯普拉格-道利大鼠：对照组（健康）、赋形剂组（诱导牙周炎）、低剂量和高剂量CBD与牛磺酸组，每组再治疗21天。获取大鼠牙齿并进行组织形态计量学研究。两者的组合显著降低了炎症标志物TNF-α和IL-1β的表达以及TRAP+细胞数量和吸收凹陷面积。在诱导牙周炎的大鼠中，用CBD和牛磺酸治疗后，牙槽骨吸收水平、牙周袋深度以及牙骨质釉质界（CEJ）与牙槽嵴顶（ABC）之间的距离显著降低，这表明将CBD与牛磺酸联合使用可能是一种治疗牙周疾病的新型治疗剂。
