Sun Jianghao, Xiao Zhenlei, Lin Long-Ze, Lester Gene E, Wang Qin, Harnly James M, Chen Pei
Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Beltsville, Maryland 20705, United States.
J Agric Food Chem. 2013 Nov 20;61(46):10960-70. doi: 10.1021/jf401802n. Epub 2013 Nov 5.
Brassica vegetables are known to contain relatively high concentrations of bioactive compounds associated with human health. A comprehensive profiling of polyphenols from five Brassica species microgreens was conducted using ultrahigh-performance liquid chromatography photodiode array high-resolution multistage mass spectrometry (UHPLC-PDA-ESI/HRMS(n)). A total of 164 polyphenols including 30 anthocyanins, 105 flavonol glycosides, and 29 hydroxycinnamic acid and hydroxybenzoic acid derivatives were putatively identified.The putative identifications were based on UHPLC-HRMS(n) analysis using retention times, elution orders, UV-vis and high-resolution mass spectra, and an in-house polyphenol database as well as literature comparisons. This study showed that these five Brassica species microgreens could be considered as good sources of food polyphenols.
已知芸苔属蔬菜含有相对较高浓度的与人类健康相关的生物活性化合物。使用超高效液相色谱光电二极管阵列高分辨率多级质谱法(UHPLC-PDA-ESI/HRMS(n))对五种芸苔属蔬菜嫩苗中的多酚进行了全面分析。共推定鉴定出164种多酚,包括30种花青素、105种黄酮醇苷以及29种羟基肉桂酸和羟基苯甲酸衍生物。推定鉴定基于UHPLC-HRMS(n)分析,利用保留时间、洗脱顺序、紫外可见光谱和高分辨率质谱,以及内部多酚数据库和文献比较。本研究表明,这五种芸苔属蔬菜嫩苗可被视为食物多酚的良好来源。