Lin Long-Ze, Sun Jianghao, Chen Pei, Zhang Ren-Wei, Fan Xiao-E, Li Lai-Wei, Harnly James M
Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Building-161, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705, United States.
J Agric Food Chem. 2014 Jul 2;62(26):6118-29. doi: 10.1021/jf405538d. Epub 2014 Jun 18.
An UHPLC-PDA-ESI/HRMS(n) profiling method was used to identify the glucosinolates and flavonoids of Rorippa indica (Cruciferae), a wild vegetable and Chinese herb used to treat cough, diarrhea, and rheumatoid arthritis. Thirty-three glucosinolates, more than 40 flavonol glycosides, and 18 other phenolic and common organic compounds were identified. The glucosinolates and polyphenols were separated by UHPLC. High-resolution deprotonated molecules provided high accuracy mass values that were used to determine formulas and provide putative identification of the glucosinolates and flavonoids. The fragments from multistage mass spectrometry were used to elucidate the structures. The concentrations of the main components were based on UV peak areas and molar relative response factors with a single calibration standard. This study found this plant to be a rich source for glucosinolates, containing 24 new glucosinolates, including 14 glucosylated glucosinolates that were previously unidentified.
采用超高效液相色谱-光电二极管阵列-电喷雾离子源/高分辨质谱（UHPLC-PDA-ESI/HRMS(n)）分析方法，对蔊菜（十字花科）中的硫代葡萄糖苷和黄酮类化合物进行了鉴定。蔊菜是一种野生蔬菜和中药材，可用于治疗咳嗽、腹泻和类风湿性关节炎。共鉴定出33种硫代葡萄糖苷、40多种黄酮醇苷以及18种其他酚类和常见有机化合物。硫代葡萄糖苷和多酚类化合物通过超高效液相色谱进行分离。高分辨率去质子化分子提供了高精度的质量值，用于确定分子式并对硫代葡萄糖苷和黄酮类化合物进行推定鉴定。多级质谱的碎片用于阐明结构。主要成分的浓度基于紫外峰面积和摩尔相对响应因子，并采用单一校准标准。本研究发现该植物是硫代葡萄糖苷的丰富来源，含有24种新的硫代葡萄糖苷，其中包括14种以前未鉴定的糖基化硫代葡萄糖苷。
