在大鼠中，含有P物质的大突触小泡和小突触小泡具有共同表面抗原的证据。

Evidence that large synaptic vesicles containing substance P and small synaptic vesicles have a surface antigen in common in rat.

作者信息

Floor E, Leeman S E

出版信息

Neurosci Lett. 1985 Sep 30;60(2):231-7. doi: 10.1016/0304-3940(85)90249-6.

Abstract

Synaptic vesicles were purified from rat brain synaptosomes by osmotic lysis and chromatography on CPG-3000 controlled-pore glass beads. Large (approximately 100 nm) synaptic vesicles containing the peptide substance P (SP) were shown to be immunoprecipitated by a monoclonal antibody previously shown by W.D. Matthew, L. Tsavaler and L.F. Reichardt (J. Cell Biol., 91 (1981) 257-269) to selectively immunoprecipitate small (approximately 50 nm) synaptic vesicles from brain. Precipitation of SP-containing vesicles showed a saturable dependence on antibody concentration. These findings constitute direct, immunochemical evidence that large, peptidergic synaptic vesicles and small synaptic vesicles from brain have a surface antigen in common.

摘要

通过渗透裂解和在CPG - 3000可控孔径玻璃珠上进行色谱分析，从大鼠脑突触体中纯化突触小泡。含有肽物质P（SP）的大（约100纳米）突触小泡被一种单克隆抗体免疫沉淀，W.D.马修、L.察瓦勒和L.F.赖夏德特（《细胞生物学杂志》，91（1981）257 - 269）先前已证明该单克隆抗体能从脑中选择性免疫沉淀小（约50纳米）突触小泡。含SP小泡的沉淀显示出对抗体浓度的饱和依赖性。这些发现构成了直接的免疫化学证据，表明脑中的大的肽能突触小泡和小突触小泡具有共同的表面抗原。