Bartling G J, Chattopadhyay S K, Barker C W, Brown H D
Can J Biochem. 1975 Aug;53(8):868-74. doi: 10.1139/o75-118.
A new method of enzyme immobilization has been described using poly(4-methacryloxybenzoic acid) as the carrier. Activation of the polymer, prior to enzyme attachment, was achieved with N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. The enzyme coupling step proceeded through nucleophilic attack by the protein on a mixed carbonic anhydride. The degree of polymer activation was determined by analysis for quinoline, a by-product of the reaction. The polymer-enzyme complex was compared to the enzyme in solution in terms of pH optimum, substrate kinetics, and thermal denaturation. Potential uses of the polymerenzyme system in chemical synthesis of benzoquinone derivatives are discussed.
已描述了一种使用聚（4-甲基丙烯酰氧基苯甲酸）作为载体的酶固定化新方法。在酶附着之前，用N-乙氧羰基-2-乙氧基-1,2-二氢喹啉实现聚合物的活化。酶偶联步骤通过蛋白质对混合碳酸酐的亲核攻击进行。通过分析反应副产物喹啉来确定聚合物的活化程度。将聚合物 - 酶复合物与溶液中的酶在最适pH、底物动力学和热变性方面进行了比较。讨论了聚合物 - 酶系统在苯醌衍生物化学合成中的潜在用途。
