Yang Tiantong, He Guanglong, Zhang Xiang, Chang Lin, Zhang Haidong, Ripple Mary G, Fowler David R, Li Ling
Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, 25 West Tucheng Road, Haidian District, Beijing, 100088, China.
J Forensic Sci. 2014 Jan;59(1):231-5. doi: 10.1111/1556-4029.12290. Epub 2013 Oct 22.
The objective of this study was to evaluate the application of Fourier transform infrared (FTIR) spectroscopy for detecting diffuse axonal injury (DAI) in a mouse model. Brain tissues from DAI mouse model were prepared with H&E, silver, and β-amyloid precursor protein (β-APP) immunohistochemistry stains and were also studied with FTIR. The infrared spectrum images showed high absorption of amide II in the subcortical white matter of the experimental mouse brain, while there was no obvious expression of amide II in the control mouse brain. The areas with high absorption of amide II were in the same distribution as the DAI region confirmed by the silver and β-APP studies. The result suggests that high absorption of amide II correlates with axonal injury. The use of FTIR imaging allows the biochemical changes associated with DAI pathologies to be detected in the tissues, thus providing an important adjunct method to the current conventional pathological diagnostic techniques.
本研究的目的是评估傅里叶变换红外（FTIR）光谱技术在检测小鼠弥漫性轴索损伤（DAI）模型中的应用。对DAI小鼠模型的脑组织进行苏木精-伊红（H&E）染色、银染色和β-淀粉样前体蛋白（β-APP）免疫组化染色，并采用FTIR进行研究。红外光谱图像显示，实验小鼠脑皮质下白质中酰胺II有高吸收，而对照小鼠脑中酰胺II无明显表达。酰胺II高吸收区域与银染色和β-APP研究证实的DAI区域分布相同。结果表明，酰胺II的高吸收与轴索损伤相关。FTIR成像的应用能够检测组织中与DAI病理相关的生化变化，从而为当前传统病理诊断技术提供一种重要的辅助方法。
