Wang Fulei, Yang Tiantong, Li Jian, Zhou Xiaowei, Liu Liang
Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, China.
Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, 25 West Tucheng Road, Haidian District, Beijing 100088, China.
Leg Med (Tokyo). 2019 Mar;37:76-82. doi: 10.1016/j.legalmed.2019.02.001. Epub 2019 Feb 2.
The diagnosis of diffuse axonal injury (DAI) is an important task in forensic pathology and clinical medicine. This study aimed to explore the use of Fourier transform infrared spectroscopy (FTIR) to detect DAI. The DAI area of the rat model was detected point by point by the FTIR-mapping system. Infrared spectral data of DAI were obtained by selecting the amide A band, CH symmetric stretching, collagen triple-helix structure and asymmetric stretching vibrational frequency of nucleic acid and phospholipid PO as the target peak positions. The system can automatically draw infrared spectral color pathological images. In the DAI group, the amide A protein secondary amine N-H stretching vibration and the collagen triple-helix structure of the high-absorption area were consistent with the DAI area confirmed by the silver and β-APP staining. The CH symmetric stretching, nucleic acid and phospholipid PO symmetric stretching vibration absorption spectra showed no significant differences between the experimental and verification groups. The FTIR-mapping technique can visually express the molecular characteristics of DAI, which is expected to be applied to the pathological diagnosis of DAI.
弥漫性轴索损伤（DAI）的诊断是法医学病理学和临床医学中的一项重要任务。本研究旨在探索利用傅里叶变换红外光谱（FTIR）检测DAI。通过FTIR映射系统逐点检测大鼠模型的DAI区域。通过选择酰胺A带、CH对称伸缩振动、胶原蛋白三螺旋结构以及核酸和磷脂PO的不对称伸缩振动频率作为目标峰位置，获取DAI的红外光谱数据。该系统可自动绘制红外光谱彩色病理图像。在DAI组中，酰胺A蛋白仲胺N-H伸缩振动和高吸收区域的胶原蛋白三螺旋结构与经银染和β-淀粉样前体蛋白（β-APP）染色确认的DAI区域一致。CH对称伸缩振动、核酸和磷脂PO对称伸缩振动吸收光谱在实验组和验证组之间无显著差异。FTIR映射技术能够直观地表达DAI的分子特征，有望应用于DAI的病理诊断。
