Beinborn M, Kromer W, Staar U, Sewing K F
Res Commun Chem Pathol Pharmacol. 1985 Sep;49(3):337-51.
Binding of 3H-iloprost was studied in a 20,000 x g sediment of the rat gastric mucosa. When pH in both test tubes for total and non-specific binding was kept identical, no displaceable binding of iloprost could be detected. When no care was taken to keep the pH identical in corresponding test tubes of the binding assay, changes in pH simulated specific and displaceable binding of iloprost. Therefore it is concluded that - in contrast to earlier reports - it is not possible to demonstrate specific iloprost binding using the given method.
在大鼠胃黏膜20,000×g沉淀物中研究了³H-伊洛前列素的结合情况。当用于总结合和非特异性结合的两个试管中的pH保持相同时，未检测到伊洛前列素的可置换结合。当在结合测定的相应试管中未注意保持pH相同时，pH的变化模拟了伊洛前列素的特异性和可置换结合。因此得出结论，与早期报告相反，使用给定方法不可能证明伊洛前列素的特异性结合。
