前列腺素对离体猪胃黏膜细胞中糖蛋白和蛋白质合成的刺激作用。
Stimulation of glycoprotein and protein synthesis in isolated pig gastric mucosal cells by prostaglandins.
作者信息
Heim H K, Oestmann A, Sewing K F
机构信息
Department of Pharmacology, School of Medicine, Hanover, W Germany.
出版信息
Gut. 1990 Apr;31(4):412-6. doi: 10.1136/gut.31.4.412.
The purpose of this study is to evaluate the effects of different prostaglandin derivatives on protein and glycoprotein synthesis and secretion in isolated and enriched pig gastric mucous cells, as measured by the incorporation of [3H]L-leucine and N-acetyl-[14C]D-glucosamine respectively into acid insoluble macromolecules (AIM). PGE2 and 16,16-dimethyl-PGE2 enhanced the incorporation of the amino sugar into cellular (EC50 8 and 75 nmol/l) and secreted (EC50 30 and 270 nmol/l) AIM in a concentration dependent manner during a 20 hours incubation. After incubation for eight hours or more they also stimulated the incorporation of [3H]L-leucine into cellular AIM. PGF2 alpha was considerably less potent (EC50 greater than 1 mumol/l) than the E-type prostaglandins. Iloprost, a stable prostacyclin analogue, was ineffective.
本研究的目的是评估不同前列腺素衍生物对分离和富集的猪胃黏液细胞中蛋白质和糖蛋白合成及分泌的影响，分别通过[3H]L-亮氨酸和N-乙酰-[14C]D-葡萄糖胺掺入酸不溶性大分子（AIM）来测定。在20小时的孵育过程中，PGE2和16,16-二甲基-PGE2以浓度依赖的方式增强了氨基糖掺入细胞内（EC50分别为8和75 nmol/L）和分泌的（EC50分别为30和270 nmol/L）AIM。孵育8小时或更长时间后，它们还刺激了[3H]L-亮氨酸掺入细胞内AIM。PGF2α的效力明显低于E型前列腺素（EC50大于1 μmol/L）。稳定的前列环素类似物伊洛前列素无效。
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