Department of Pediatrics, Botucatu Medical School - Sao Paulo State University (UNESP), Botucatu, SP, Brazil; Jean Mayer USDA - Human Nutrition Research Center on Aging, Tufts University, Boston, MA, USA; Department of Health Sciences - Universidade do Sagrado Coração (USC), Bauru, SP, Brazil.
Respir Physiol Neurobiol. 2014 Jan 1;190:118-23. doi: 10.1016/j.resp.2013.10.008. Epub 2013 Oct 19.
To compare conventional mechanical ventilation (CMV) and high-frequency oscillatory ventilation (HFOV), with/without inhaled nitric oxide (iNO), for oxygenation, inflammation, antioxidant/oxidative stress status, and DNA damage in a model of acute lung injury (ALI). Lung injury was induced by tracheal infusion of warm saline. Rabbits were ventilated at [Formula: see text] 1.0 and randomly assigned to one of five groups. Overall antioxidant defense/oxidative stress was assessed by total antioxidant performance assay, and DNA damage by comet assay. Ventilatory and hemodynamic parameters were recorded every 30min for 4h. ALI groups showed worse oxygenation than controls after lung injury. After 4h of mechanical ventilation, HFOV groups presented significant improvements in oxygenation. HFOV with and without iNO, and CMV with iNO showed significantly increased antioxidant defense and reduced DNA damage than CMV without iNO. Inhaled nitric oxide did not beneficially affect HFOV in relation to antioxidant defense/oxidative stress and pulmonary DNA damage. Overall, lung injury was reduced using HFOV or CMV with iNO.
比较常规机械通气（CMV）和高频振荡通气（HFOV），联合/不联合吸入一氧化氮（iNO），在急性肺损伤（ALI）模型中用于氧合、炎症、抗氧化/氧化应激状态和 DNA 损伤。通过气管内输注温盐水诱导肺损伤。兔子以[Formula: see text]1.0 通气，并随机分为五组之一。通过总抗氧化能力测定法评估整体抗氧化防御/氧化应激，通过彗星试验评估 DNA 损伤。通气和血流动力学参数每 30min 记录一次，共 4h。ALI 组在肺损伤后氧合比对照组差。在机械通气 4h 后，HFOV 组的氧合明显改善。与 CMV 组相比，HFOV 联合和不联合 iNO，以及 CMV 联合 iNO 显示出明显增加的抗氧化防御和减少的 DNA 损伤。吸入一氧化氮对 HFOV 在抗氧化防御/氧化应激和肺 DNA 损伤方面没有有益影响。总的来说，使用 HFOV 或 CMV 联合 iNO 可以减轻肺损伤。
