Karavanova I D, Bannikov G A, Troianovskiĭ S M
Tsitologiia. 1985 Sep;27(9):1039-42.
Results obtained by the indirect immunofluorescence method employing specific monoclonal antibodies show that during the first 24 hours of cultivation in a monolayer there appears another protein of intermediate filaments--vimentin, which is a characteristic of most mesenchymal cells. At the same time, in the organ liver culture maintained in the same culture medium, no expression of vimentin was observed up to 5-7 days of cultivation. Vimentin was revealed only in cells that migrated from a tissuepiece to collagen. Besides the vimentin expression in these migrating cells and monolayer cultures of hepatocytes, a redistribution of prekeratin filaments took place: the cytoplasmic network appeared instead of thick fibers underlying membranes. The results of the present work suggest that the vimentin expression and the prekeratin filament redistribution in epithelial liver cells in vitro do not depend on the changes of natural humoral factors for the components of culture medium but are due to damages of the intact liver tissue structure.
采用特异性单克隆抗体的间接免疫荧光法所获得的结果表明，在单层培养的最初24小时内，出现了另一种中间丝蛋白——波形蛋白，这是大多数间充质细胞的特征。与此同时，在相同培养基中维持的器官肝脏培养物中，直到培养5 - 7天，均未观察到波形蛋白的表达。波形蛋白仅在从组织块迁移至胶原的细胞中被发现。除了这些迁移细胞和肝细胞单层培养物中有波形蛋白表达外，前角蛋白丝还发生了重新分布：出现了细胞质网络，而非位于膜下的粗纤维。本研究结果表明，体外培养的肝上皮细胞中波形蛋白的表达和前角蛋白丝的重新分布并不取决于培养基成分中天然体液因子的变化，而是由于完整肝组织结构的损伤。
