Chipysheva T A, Gel'shteĭn V I, Troianovskiĭ S M, Bannikov G A
Ontogenez. 1988 Mar-Apr;19(2):138-42.
Monoclonals against three individual proteins of the epithelial intermediate filaments, prekeratins (PK40, PK49, PK55), were used for immunofluorescence studies of the cryostat sections of the rat embryonic liver. The dynamics of expression and intracellular distribution of prekeratins reflects that of morphological rearrangement of the liver. The development of the system of liver beams was accompanied by changes in the expression and intracellular distribution of PK49 and PK55 and the development of the system of bile ducts by changes in all three PKs. From day 20-21 of embryogenesis all three PKs are expressed in cholangiocytes, while PK49 and PK55 in hepatocytes only.
针对上皮中间丝的三种单个蛋白质（前角蛋白，PK40、PK49、PK55）的单克隆抗体，被用于大鼠胚胎肝脏冰冻切片的免疫荧光研究。前角蛋白的表达动态和细胞内分布反映了肝脏形态重排的动态。肝板系统的发育伴随着PK49和PK55表达及细胞内分布的变化，而胆管系统的发育则伴随着所有三种前角蛋白的变化。从胚胎发育的第20 - 21天起，所有三种前角蛋白均在胆管细胞中表达，而PK49和PK55仅在肝细胞中表达。
