Laboratório de Biologia de Helmintos Otto Wucherer, Centro de Ciências da Saúde, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Bloco G, Ilha do Fundão, 21949-900 Rio de Janeiro, Brazil.
Microsc Microanal. 2013 Dec;19(6):1470-4. doi: 10.1017/S1431927613013706. Epub 2013 Oct 22.
Morphological studies of helminths by scanning electron microscopy are generally limited to the external topography of the organisms. In this work, we present a simple technique using ethanol as a cryoprotectant without postfixation in osmium tetroxide that allows for observation of the inner organization of helminths and preserves cellular structures. We tested the technique in three helminths: Echinostoma paraensei, Cruzia tentaculata, and Hassalstrongylus epsilon. The results show that this technique could potentially be applied to study the morphology, ultrastructure, and taxonomy of helminths.
扫描电子显微镜下的寄生虫形态学研究通常仅限于生物体的外部形貌。在这项工作中，我们提出了一种简单的技术，使用乙醇作为冷冻保护剂，而无需在锇酸中进行后固定，从而可以观察寄生虫的内部组织并保留细胞结构。我们在三种寄生虫中测试了该技术：Paraensei 棘口吸虫、毛首鞭形线虫和ε哈氏副柔线虫。结果表明，该技术可能适用于研究寄生虫的形态、超微结构和分类学。
