Public Health England (Colindale), London.
Clin Infect Dis. 2014 Jan;58(2):181-7. doi: 10.1093/cid/cit692. Epub 2013 Oct 22.
We report on a follow-up clinical and serological investigation of 274 children who received seasonal influenza vaccine (trivalent inactivated vaccine [TIV]) 1 year after receipt of either AS03(B)-adjuvanted subunit or whole virus monovalent A(H1N1)pdm09 vaccine and describe the antibody responses to the H3N2 A/Perth/16/2009 and B/Brisbane/60/2008 components of TIV.
Vaccine responses were analyzed using hemagglutination inhibition (HAI) assays. In children aged <3 years, previous receipt of adjuvanted vaccine resulted in higher HAI antibody responses to H3N2 and B strains compared with nonadjuvanted vaccine (fold change 16.8 vs 4.3 for H3N2 and 7.0 vs 1.6 for B). In children aged >3 years, responses to the H3 and B components of TIV were similar between vaccine groups. Sera taken before and after the pandemic vaccine were also analyzed by HAI using A/Perth/16/2009 virus. This analysis showed that 11.1% of children receiving the AS03(B)-adjuvanted vaccine but only 1.4% in the nonadjuvanted group had a 4-fold rise to A/Perth/16/2009.
AS03B-adjuvanted A(H1N1)pdm09 influenza vaccine generates a cross-reactive antibody response to H3N2 in children and enhances responses to heterologous subtypes in children aged <3 years 1 year later.
我们报告了 274 名儿童在接种季节性流感疫苗（三价灭活疫苗[TIV]）1 年后的临床和血清学随访结果，这些儿童此前分别接种了 AS03（B）-佐剂亚单位或全病毒单价 A（H1N1）pdm09 疫苗。我们描述了 TIV 中 H3N2 A/Perth/16/2009 和 B/Brisbane/60/2008 成分的抗体反应。
使用血凝抑制（HAI）试验分析疫苗反应。在年龄<3 岁的儿童中，与非佐剂疫苗相比，先前接种佐剂疫苗导致对 H3N2 和 B 株的 HAI 抗体反应更高（H3N2 的倍数变化为 16.8 比 4.3，B 为 7.0 比 1.6）。在年龄>3 岁的儿童中，疫苗组之间对 TIV 的 H3 和 B 成分的反应相似。还通过 HAI 使用 A/Perth/16/2009 病毒分析了大流行疫苗前后采集的血清。该分析表明，接受 AS03（B）-佐剂疫苗的儿童中有 11.1%，而未接受佐剂疫苗的儿童中仅有 1.4%对 A/Perth/16/2009 出现 4 倍增长。
AS03B 佐剂 A（H1N1）pdm09 流感疫苗可在儿童中产生针对 H3N2 的交叉反应性抗体反应，并增强 3 岁以下儿童对异源亚型的反应 1 年后。
