Mirkes P E
Anal Biochem. 1985 Aug 1;148(2):376-83. doi: 10.1016/0003-2697(85)90242-8.
A procedure for the simultaneous banding of cellular DNA, RNA, and protein by centrifugation in cesium trifluoroacetate (CsTFA) gradients is described. Starting with homogenates of Day 11 rat embryos, this procedure was used to separate total DNA, RNA, and protein. Under the conditions used DNA banded at a peak density of 1.63 g/ml, RNA at a peak density of 1.83 g/ml, and protein at a peak density of 1.40 g/ml. Nucleic acids isolated from CsTFA gradients were judged to be protein free. RNA isolated by this method is apparently free of DNA contamination; however, DNA isolated by this method does contain some RNA (less than 5% contamination).
本文描述了一种通过在三氟乙酸铯（CsTFA）梯度中离心同时对细胞DNA、RNA和蛋白质进行分带的方法。从第11天大鼠胚胎的匀浆开始，该方法用于分离总DNA、RNA和蛋白质。在所使用的条件下，DNA在峰值密度1.63 g/ml处形成条带，RNA在峰值密度1.83 g/ml处形成条带，蛋白质在峰值密度1.40 g/ml处形成条带。从CsTFA梯度中分离出的核酸被判定无蛋白质。通过该方法分离的RNA显然没有DNA污染；然而，通过该方法分离的DNA确实含有一些RNA（污染小于5%）。
