稳定的 Wittig 烯烃加成法用于生物缀合。
Stabilized Wittig olefination for bioconjugation.
机构信息
Division of Organic Chemistry, Institute of Chemical and Engineering Sciences, Agency for Science Technology and Research Singapore, 11 Biopolis Way, Helios Block #03-08, Singapore.
出版信息
Chem Commun (Camb). 2013 Dec 11;49(95):11188-90. doi: 10.1039/c3cc45961f. Epub 2013 Oct 23.
PMID:24150718
Abstract
Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP12.
摘要
稳定的 Wittig 烯烃加成反应具有作为生物偶联反应的巨大潜力。我们证明，稳定的磷叶立德（或鏻盐）与芳基醛的反应足够稳健，可用于活细胞亲和分离和蛋白质 FKBP12 的荧光标记。
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