土耳其东部儿童中幽门螺杆菌毒力基因型的流行情况。
Prevalence of Helicobacter pylori virulence genotypes among children in Eastern Turkey.
机构信息
Gokben Ozbey, Vocational School of Health Services, Firat University, 23119, Elazig, Turkey.
出版信息
World J Gastroenterol. 2013 Oct 21;19(39):6585-9. doi: 10.3748/wjg.v19.i39.6585.
AIM
To identify the virulence genotypes of Helicobacter pylori (H. pylori) if present in children in Eastern Turkey and if those genotypes are mostly associated with severe clinical presentations.
METHODS
A total of 49 H. pylori positive Turkish children (42 with antral nodularity and 7 with peptic ulcer) who underwent upper gastrointestinal endoscopy with abdominal symptoms during the period from March 2011 to September 2012 were enrolled in this study. Antral nodularity was diagnosed endoscopically by two of the authors. We determined for the presence of cagA, vacA, cagE, iceA and babA2 genotypes of H. pylori isolates in DNA obtained directly from frozen gastric biopsy samples by polymerase chain reaction test using specific primers.
RESULTS
Of the 49 H. pylori isolates studied, 61.2%, 91.8%, 22.4%, 28.6%, 57.1% and 40.8% were positive for the cagA, vacA s1, cagE, iceA1, iceA2 and babA2 genes, respectively. We showed that the most common vacA subtype was s1a (79.6%). However, the s2 gene was found less frequently with an isolation rate of 8.2% of the H. pylori isolates. The genotypes iceA2 and vacA s1m2 were the most frequently found types in children with antral nodularity. In addition, the genotypes iceA1, babA2 and vacA s1m1 were found in similar ratios in all the H. pylori isolates obtained from children with peptic ulcer. The genotypes vacA s2m1 and s1c were not observed in any of isolates studied.
CONCLUSION
This study showed that vacA s1m2, cagA and iceA2 were the most common genotypes, and no association between antral nodularity and genotypes was observed.
目的
确定在土耳其东部的儿童中是否存在幽门螺杆菌（H. pylori）的毒力基因型，如果存在，这些基因型是否与严重的临床表型密切相关。
方法
本研究共纳入了 49 例土耳其儿童（42 例有胃窦结节，7 例有消化性溃疡），他们在 2011 年 3 月至 2012 年 9 月期间因腹部症状接受了上消化道内镜检查。两名作者通过内镜诊断胃窦结节。我们通过聚合酶链反应试验，使用特定的引物，直接从冷冻胃活检样本中获得的 DNA 中确定了 H. pylori 分离株中 cagA、vacA、cagE、iceA 和 babA2 基因型的存在。
结果
在研究的 49 例 H. pylori 分离株中，cagA、vacA s1、cagE、iceA1、iceA2 和 babA2 基因的阳性率分别为 61.2%、91.8%、22.4%、28.6%、57.1%和 40.8%。我们发现最常见的 vacA 亚型是 s1a（79.6%）。然而，s2 基因的分离率较低，为 8.2%。在胃窦结节患儿中，iceA2 和 vacA s1m2 是最常见的基因型。此外，在所有来自消化性溃疡患儿的 H. pylori 分离株中，发现 iceA1、babA2 和 vacA s1m1 的基因型比例相似。在研究的所有分离株中均未观察到 vacA s2m1 和 s1c 基因型。
结论
本研究表明，vacA s1m2、cagA 和 iceA2 是最常见的基因型，胃窦结节与基因型之间没有相关性。
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