Department of Medical Microbiology, Medical University of Sofia, 1431 Sofia, Bulgaria.
Antonie Van Leeuwenhoek. 2010 Oct;98(3):343-50. doi: 10.1007/s10482-010-9448-y. Epub 2010 May 8.
Data on the geographic prevalence of Helicobacter pylori iceA and babA alleles in Eastern Europe are still relatively scant. The aim of this study was to evaluate the prevalence of iceA and babA genotypes in Bulgarian symptomatic patients. The iceA and babA genotypes were evaluated by PCR with pure cultures in strains from 196 and 181 patients, respectively. Mixed infections were found in 10.2% of all 196 patients. Prevalence of H. pylori genotypes in patients with single-strain infections was 69.3% for iceA1, 30.7% for iceA2, 82.4% for cagA(+), 89.2% for vacA s1, 10.8% for vacA s2, 39.8% for vacA m1, 60.2% for vacA m2 and 48.8% for babA2. Within the iceA1 positive strains, 94.3% and 88.5% were also vacA s1a and cagA positive, respectively. Of the babA2 positive strains, 100.0%, 92.4% and 72.2% were also vacA s1a, cagA and iceA1 positive, respectively. Ulcer patients had more often strains with cagA positive status and vacA s1a allele. Although neither iceA1 nor babA2 were more common in ulcer patients, the combination of both alleles was more frequent (48.1%) in the ulcer patients than in the rest (28.7%). Clarithromycin susceptible strains had more often iceA1 allele (74.4%) than the resistant strains (55.3%). In conclusion, the results demonstrated a high prevalence of virulent H. pylori in Bulgaria. Both iceA1 and babA2 genotypes were associated with other virulence factors of H. pylori and, in addition, the iceA1 allele was associated with the strain susceptibility.
东欧有关幽门螺杆菌冰 A 和 babA 等位基因的地理流行数据仍然相对较少。本研究的目的是评估保加利亚有症状患者中冰 A 和 babA 基因型的流行情况。通过对 196 例和 181 例患者的纯培养物进行 PCR，评估了冰 A 和 babA 基因型。在所有 196 例患者中，发现混合感染占 10.2%。在单株感染患者中，H. pylori 基因型的流行率为冰 A1 为 69.3%，冰 A2 为 30.7%，cagA(+)为 82.4%，vacA s1 为 89.2%，vacA s2 为 10.8%，vacA m1 为 39.8%，vacA m2 为 60.2%，babA2 为 48.8%。在冰 A1 阳性菌株中，vacA s1a 和 cagA 阳性率分别为 94.3%和 88.5%。在 babA2 阳性菌株中，vacA s1a、cagA 和冰 A1 阳性率分别为 100.0%、92.4%和 72.2%。溃疡患者的菌株更常具有 cagA 阳性状态和 vacA s1a 等位基因。尽管冰 A1 和 babA2 在溃疡患者中并不更常见，但两者的组合在溃疡患者中更为常见（48.1%），而在其余患者中则更为常见（28.7%）。克拉霉素敏感菌株的冰 A1 等位基因（74.4%）比耐药菌株（55.3%）更为常见。总之，结果表明保加利亚存在高毒力的幽门螺杆菌。冰 A1 和 babA2 基因型均与幽门螺杆菌的其他毒力因子相关，此外，冰 A1 等位基因与菌株的敏感性相关。
