a Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University , Iksan , South Korea.
Prep Biochem Biotechnol. 2014;44(2):119-31. doi: 10.1080/10826068.2013.792277.
Protease production by Bacillus sp. SKK11 isolated from brackish environment was studied by solid-state fermentation with horse gram husk. Response surface methodology-based Box-Behnken design (BBD) was used to optimize the variables such as pH, maltose, and MgSO₄. The BBD design analysis showed a reasonable adjustment of the quadratic model with the experimental data. Statistics-based contour and three-dimensional (3-D) plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and the culture conditions. The maximum yield of the enzyme was observed at pH 9.0.
从咸水环境中分离出的芽孢杆菌 SKK11 通过固态发酵马豆壳生产蛋白酶。采用基于响应面法的 Box-Behnken 设计（BBD）优化了 pH、麦芽糖和 MgSO₄ 等变量。BBD 设计分析表明，该二次模型能够合理地调整实验数据。基于统计学的等高线和三维（3-D）图生成，以评估响应面的变化，并了解酶产量与培养条件之间的关系。在 pH 9.0 时观察到酶的最大产量。
