通过间接竞争酶联免疫吸附测定法检测癌症患者血清样本中的白色念珠菌Sap2用于念珠菌病的诊断。
Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis.
作者信息
Wang Yicun, Gao Xiang, Zhi Gang J U, Liu Jingyuan, Dong Shuai, Wang Li
机构信息
School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province, P.R. China, .
出版信息
Indian J Pathol Microbiol. 2013 Jul-Sep;56(3):243-7. doi: 10.4103/0377-4929.120377.
BACKGROUND
The secreted aspartyl proteinases 2 (Sap2) of Candida albicans (C. albicans) is a potential marker of candididasis. It is a virulence factor associated with adherence and tissue invasion.
AIM
In order to detect Sap2 in clinical sera, we developed an indirect competitive enzyme-linked immunosorbent assay (ELISA).
MATERIALS AND METHODS
Polyclonal antibodies were produced for Sap2 by injecting Sap2 into a New Zealand White inbred rabbit. They could be used at a dilution exceeding 1:1200 in an indirect ELISA, and detected Sap2 concentration up to 1 ng/mL.
RESULTS
Of the 286 cancer serum samples tested, 16.8% were found as candidiasis. The test was simple and economical to perform and had a level of sensitivity for detection of low-titer positive sera; thus, it may be proven to be of value in epidemiological studies on candidiasis.
背景
白色念珠菌的分泌天冬氨酸蛋白酶2（Sap2）是念珠菌病的一个潜在标志物。它是一种与黏附和组织侵袭相关的毒力因子。
目的
为了检测临床血清中的Sap2，我们开发了一种间接竞争酶联免疫吸附测定（ELISA）法。
材料与方法
通过将Sap2注射到新西兰白色近交系兔体内制备针对Sap2的多克隆抗体。它们在间接ELISA中可在超过1:1200的稀释度下使用，并且能够检测到高达1 ng/mL的Sap2浓度。
结果
在所检测的286份癌症血清样本中，发现16.8%存在念珠菌病。该检测操作简单且经济，对低滴度阳性血清的检测具有一定灵敏度；因此，它可能在念珠菌病的流行病学研究中被证明具有价值。
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