Raymondjean M, Defer N, Tichonicky L, Kruh J, Kneip B, Guguen-Guillouzo C
Carcinogenesis. 1985 Dec;6(12):1797-803. doi: 10.1093/carcin/6.12.1797.
Hepatocarcinoma was induced by administration of diethylnitrosamine to rats. The rats were sacrificed 70 weeks after the administration and the carcinoma nodules were separated from the perinodular parenchymental cells after perfusion of liver with collagenase. The in vitro translational pattern of mRNAs from hepatocellular carcinomas, from perinodular hepatocytes and from regenerating liver after partial hepatectomy were compared by one- and two-dimensional electrophoreses to the pattern obtained with RNA from normal hepatocytes. An increased synthesis of several peptides was observed with RNAs from carcinoma and from regenerating liver and to a lesser extent with RNA from perinodular hepatocytes, which suggests that the increase in synthesis is at least partly related to cell proliferation. A decreased synthesis of several other peptides was observed with RNA from carcinoma nodules and to a lesser extent with RNA from perinodular hepatocytes, but not with RNA from regenerating liver, which suggests that this decrease in synthesis is related to some transformation specific process. These changes are observed as soon as 22 weeks after carcinogen administration. These observations also suggest that at least part of the perinodular hepatocytes have some characteristics of the transformed cells.
通过给大鼠注射二乙基亚硝胺诱导肝癌。给药70周后处死大鼠，用胶原酶灌注肝脏后，将癌结节与结节周围实质细胞分离。通过一维电泳和二维电泳，比较了肝癌细胞、结节周围肝细胞以及部分肝切除后再生肝的mRNA体外翻译模式与正常肝细胞RNA的翻译模式。癌组织和再生肝的RNA观察到几种肽的合成增加，结节周围肝细胞的RNA合成增加程度较小，这表明合成增加至少部分与细胞增殖有关。癌结节的RNA观察到其他几种肽的合成减少，结节周围肝细胞的RNA合成减少程度较小，但再生肝的RNA未观察到这种情况，这表明这种合成减少与某些转化特异性过程有关。在给予致癌物后22周就观察到了这些变化。这些观察结果还表明，至少部分结节周围肝细胞具有转化细胞的一些特征。
