Barnes D B, Pierce G F, Lichti D, Landt M, Koenig J, Chan K M
Clin Chem. 1985 Dec;31(12):2018-9.
We evaluated the effect of dextran on values for total protein in serum as measured by the biuret method with five widely used automated instruments: the American Monitor Parallel; the Du Pont aca II; the Roche Cobas-Bio; the Kodak Ektachem 400; and the Beckman Astra 8. Dextran concentrations as great as 25 or 30 g/L had relatively little or no influence on total protein measurements by the latter three instruments. Dextran concentrations exceeding 6 g/L caused falsely low results with the aca, whereas the Parallel gave falsely high results when the dextran concentration exceeded 2 g/L. The aca total protein procedure could be protected from the interference by dextran concentrations up to 30 g/L by injecting 0.4-0.8 mL of ethylene glycol directly into the reagent pack before sampling. However, we could not eliminate the interference with the Parallel procedure by any simple means; we thus recommend that it not be used for measuring total protein in serum samples from patients who are being treated with dextran.
我们使用五种广泛应用的自动分析仪，通过双缩脲法评估了右旋糖酐对血清总蛋白测定值的影响，这五种仪器分别是：美国Monitor Parallel；杜邦aca II；罗氏Cobas - Bio；柯达Ektachem 400；以及贝克曼Astra 8。高达25或30 g/L的右旋糖酐浓度对后三种仪器测定总蛋白的结果影响相对较小或无影响。当右旋糖酐浓度超过6 g/L时，aca会得出错误的低结果，而当右旋糖酐浓度超过2 g/L时，Parallel会得出错误的高结果。在采样前直接向试剂包中注入0.4 - 0.8 mL乙二醇，可使aca总蛋白检测程序免受高达30 g/L右旋糖酐浓度的干扰。然而，我们无法通过任何简单方法消除Parallel程序的干扰；因此我们建议，对于正在接受右旋糖酐治疗患者的血清样本，不要使用该程序来测定总蛋白。
