Department of Laboratory Diagnostics, II Chair of Internal Medicine, Medical University of Lodz, Poland.
J Biol Regul Homeost Agents. 2013 Jul-Sep;27(3):681-92.
The CD8+CD28- and CD8+CD28+ T cells play a primordial role in peripheral tolerance, but little is known about their implication in allergic asthma. This study was designed to determine the changes in a proportion of human circulatory CD8+ subsets before and after short term culture in the presence of anti-CD3/CD28 and IL-10 or TGF-beta. Flow cytometry analysis revealed increased percentage of CD8+CD28- T cells but decreased percentage of CD8+CD28+ T cells enriched from peripheral blood of adult allergic asthma individuals compared to controls (baseline). In comparison to the baseline, co-stimulation with anti-CD3/CD28 and IL-10 decreased the proportion of CD8+CD28- T cells in severe allergic asthma subjects, whereas it increased this value in mild to moderate asthmatic subjects and controls. Adding TGF-beta decreased the proportion of CD8+CD28- T cells from allergic asthma subjects, whereas it has opposite effects on this subset from controls. IL-10 and TGF-beta had some plethoric effects on FoxP3 expression in anti-CD3/CD28 activated CD8+CD28- T cells. Thus, these findings indicate that a control mechanism involving IL-10 and TGF-beta might be defective in allergic asthma subjects.
CD8+CD28- 和 CD8+CD28+ T 细胞在外周耐受中起主要作用，但它们在过敏性哮喘中的作用知之甚少。本研究旨在确定在存在抗 CD3/CD28 和 IL-10 或 TGF-β的情况下，人类循环 CD8+亚群的比例在短期培养前后的变化。流式细胞术分析显示，与对照（基线）相比，成人过敏性哮喘个体外周血中 CD8+CD28- T 细胞的百分比增加，而 CD8+CD28+ T 细胞的百分比减少。与基线相比，抗 CD3/CD28 和 IL-10 的共刺激降低了严重过敏性哮喘患者中 CD8+CD28- T 细胞的比例，而在轻度至中度哮喘患者和对照中则增加了该值。添加 TGF-β 可降低过敏性哮喘患者中 CD8+CD28- T 细胞的比例，而对对照中该亚群则有相反的作用。IL-10 和 TGF-β 对抗 CD3/CD28 激活的 CD8+CD28- T 细胞中 FoxP3 表达有一些充盈作用。因此，这些发现表明涉及 IL-10 和 TGF-β 的控制机制可能在过敏性哮喘患者中存在缺陷。
