Radhakrishnakartha Harikrishnan, Appu Abhilash Puthuvelvippel, Madambath Indira
J Basic Clin Physiol Pharmacol. 2014 Feb;25(1):117-24. doi: 10.1515/jbcpp-2012-0056.
Chronic ethanol exposure causes hyperlipidemia. The present study was designed to investigate the impact of ascorbic acid supplementation on ethanol induced hyperlipidemia in testis and to compare it with that of abstinence from taking alcohol.
Thirty-six male guinea pigs were divided into two groups and were maintained for 90 days as follows (1) control (C) (2) ethanol treated group (E) (4 g/kg body wt/day). Ethanol was administered for 90 days and on 90th day, alanine amino transaminase (ALT), aspartate amino transaminase (AST) and γ-glutamyltransferase (GGT) in serum was assayed. The animals in the ethanol group were further divided into an ascorbic acid supplemented group (25 mg/100 g body wt/day) (E+AA) and an ethanol abstention group (EAG) and those in the control group were divided into a control group and a control+ascorbic acid group (C+AA).
There was significant increase in levels of testicular cholesterol, free fatty acid, phospholipids and triglycerides in the ethanol group. There was also a significant increase in the activity of HMG CoA reductase and decrease in activity of testicular glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme in ethanol-ingested animals that further led to decreased levels of serum testosterone. Alcohol administration also enhanced the activity of testicular alcohol dehydrogenase (ADH). Ascorbic acid supplementation and abstention altered all these parameters induced by chronic alcohol administration. Histological studies were also in line with the above results.
Ascorbic acid was able to reinstate the cholesterol homeostasis in testis which could have further restored the testicular steroidogenesis. The present study demonstrated that ascorbic acid is effective in reducing the hyperlipidemia induced by chronic alcohol administration and produced a better recovery than abstention.
长期乙醇暴露会导致高脂血症。本研究旨在调查补充抗坏血酸对乙醇诱导的睾丸高脂血症的影响，并将其与戒酒的影响进行比较。
将36只雄性豚鼠分为两组，按以下方式饲养90天：（1）对照组（C）；（2）乙醇处理组（E）（4克/千克体重/天）。给予乙醇90天，并在第90天测定血清中的丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和γ-谷氨酰转移酶（GGT）。乙醇组的动物进一步分为抗坏血酸补充组（25毫克/100克体重/天）（E+AA）和乙醇戒断组（EAG），对照组的动物分为对照组和对照+抗坏血酸组（C+AA）。
乙醇组睾丸胆固醇、游离脂肪酸、磷脂和甘油三酯水平显著升高。摄入乙醇的动物中，3-羟基-3-甲基戊二酰辅酶A还原酶（HMG CoA reductase）活性也显著增加，而睾丸葡萄糖-6-磷酸脱氢酶（G6PDH）和苹果酸酶活性降低，这进一步导致血清睾酮水平降低。给予酒精还增强了睾丸乙醇脱氢酶（ADH）的活性。补充抗坏血酸和戒酒改变了慢性酒精给药诱导的所有这些参数。组织学研究也与上述结果一致。
抗坏血酸能够恢复睾丸中的胆固醇稳态，这可能进一步恢复睾丸类固醇生成。本研究表明，抗坏血酸可有效降低慢性酒精给药诱导的高脂血症，且比戒酒产生更好的恢复效果。
