Bliss D J, Brewer D B
Clin Sci (Lond). 1985 Sep;69(3):321-6. doi: 10.1042/cs0690321.
Female Wistar rats were made heavily proteinuric by daily intraperitoneal injections of bovine serum albumin over 5 days. The size and charge permeability characteristics of the glomerular filter in this condition were determined by studying the renal handling of poly-dispersed uncharged dextran over the range of molecular radii 2-6 nm (albumin 3.6 nm), and of endogenous rat serum albumin over the same clearance period. In proteinuric rats the clearance of rat serum albumin was significantly increased with a resultant reduction in the circulating levels of rat serum albumin. The clearance of uncharged dextran was not significantly different in proteinuric rats compared with control animals. There does not appear to be any size selective defect of the glomerular filtration barrier in this condition. This suggests that the observed increase in the clearance of the negatively charged endogenous albumin may be due to a reduction in the glomerular charge barrier.
通过连续5天每日腹腔注射牛血清白蛋白，使雌性Wistar大鼠出现严重蛋白尿。通过研究在分子半径2 - 6纳米（白蛋白为3.6纳米）范围内多分散性不带电荷右旋糖酐以及在相同清除期内内源性大鼠血清白蛋白的肾脏处理情况，来确定这种情况下肾小球滤过器的大小和电荷通透性特征。在蛋白尿大鼠中，大鼠血清白蛋白的清除率显著增加，导致大鼠血清白蛋白的循环水平降低。与对照动物相比，蛋白尿大鼠中不带电荷右旋糖酐的清除率没有显著差异。在这种情况下，肾小球滤过屏障似乎不存在任何大小选择性缺陷。这表明观察到的带负电荷内源性白蛋白清除率增加可能是由于肾小球电荷屏障降低所致。
