Madej Janusz A, Madej Jan P, Dziegiel Piotr, Pula Bartosz, Nowak Marcin
Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw 50-375, Poland.
Acta Vet Scand. 2013 Oct 24;55(1):73. doi: 10.1186/1751-0147-55-73.
The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation. Therefore, paraffin sections of 15 adenomas and 64 adenocarcinomas sampled from 79 dogs aged 6 to 16 years were analysed.
A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004). Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).
Results of this study indicate a similar biological role of HIF-1α in dogs and in humans, which may confirm suitability of the animal model in investigations on progression of tumours in humans.
本研究旨在检测母犬腺癌和腺瘤中缺氧诱导因子（HIF）1α的表达情况，及其与肿瘤恶性程度、增殖、凋亡和血管生成的关系。因此，对79只6至16岁犬的15个腺瘤和64个腺癌石蜡切片进行了分析。
与腺瘤相比，腺癌中HIF-1α表达显著更高（P < 0.0004）。此外，腺癌中HIF-1α表达与肿瘤恶性程度（r = 0.59，P < 0.05）、Ki-67抗原表达（r = 0.43；P < 0.0005）、TUNEL阳性细胞（r = 0.62，P < 0.0001）以及通过对表达血管性血友病因子的血管进行定量测量的肿瘤血管生成（r = 0.57，P < 0.05）呈正相关。
本研究结果表明HIF-1α在犬和人类中具有相似的生物学作用，这可能证实了该动物模型在人类肿瘤进展研究中的适用性。
